416
HORST ZITZELSBERGER, BENJAMIN O'BRIEN, and HEINZ-ULRICH WEIER
pended in 1xxTE buffer and quantitated by Hoechst fluorometry using a
Hoefer TK 100 instrument. Probes used in the CR 10 rainbow set are listed
in Table 1. The probe labeling and detection scheme is summarized in
Table 2.
Table 1. Composition of the probe set for chromosome 10 rainbow hybridization (CR1O)
Probe Name ProbeType
Cytogenetic
Gene or
Fractional
Probe Label
Fluorochrome
Location
Marker
Length
Emission
78 E 1
PI
10p15
DlOS249
0.017
Dig
Red
91 A 2
PI
lOp14
DlOS547
0.097
Bio
Infrared
81 G 1
PI
1Op13
DlOS603
0.154
FITC
Green
87 G 10
PI
lOpl2
DlOS588
0.214
Dig+FITC
Red and green
98 D 7
PI
lOp11.2
DlOS578
0.298
Dig
Red
58 C 11
PI
lOql1.2
Ret
0.401
Bio
Infrared
101 C 7
PI
10q21
DI0S539
0.443
FITC
Green
29 F 6
PI
10q21
DlOS170
0.478
Dig+FITC
Red and green
106 H 5
PI
lOq22
DlOS537
0.541
Dig
Red
51 H 2
PI
lOq22-23
DlOS607
0.601
Bio
Infrared
60 D 11
PI
lOq23
DlOS541
0.671
FITC
Green
66 E 12
PI
lOq24.1
NFkB-2
0.742
Dig+FITC
Red and green
911 D 9
YAC
lOq24.3
DI0S597
0.812
Dig
Red
858 H 7
YAC
lOq25.2
DlOS533
0.871
Bio
Infrared
937 A 6
YAC
lOq25-26
DlOS209
0.923
FITC
Green
932 F 11
YAC
lOq26
DlOS217
0.981
Dig+FITC
Red and green
Table 2. Probe detection scheme
Label
Layer 1
Layer 2
Fluorescence color
Biotin
Avidin-Cy-5
infrared
Digoxigenin
Rhodamine-labeled
red
sheep-anti-digoxigenin
Fluorescein
Mouse-anti-FITC FITC-labeled goatgreen
anti-mouse