31 Multicolor FISH Techniques for the Detection of Inter- and Intrachromosomal Rearrangements
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- Cititluor AF 1 or Vectashield mounting media
- con DNA solution, 1 mg/ml (Life Technologies)
- DAPI (4,6-diamidino-2-phenylindole) (Sigma)
- Distilled and sterile water
- Digoxigenin-ll-dUTP, 1 mM solution (Roche Molecular Biochemicals)
- Ethanol, p.a. (Merck)
- Fluorescein-12-dUTP, 1 mM solution (Roche Molecular Biochemicals)
- Formamide, molecular biology grade (Sigma)
- Glacial acetic acid (Merck)
- Glass Coplin jars
- Glass coverslips (22 x 22 mm)
- HCl (2M)
- Methanol, p.a. (Merck)
- Microscope slides
- NaCl
- Nonidet P40
- ParafIlm
- Phenol:chloroform:isoamyl alcohol (2S:24:I) (Sigma)
- Rabbit anti-FITC antibodies (DAKO)
- Rubber cement
- Salmon sperm DNA solution, 10 mg/ml (Life Technologies)
- Sodium citrate
- Tween 20
- Antifading solution: 0.1 % p-phenylenediamine dihydrochloride (Sigma), 0.1 xPBS (Gibco), 4S mM NaHC0 3 , 82% glycerol, pH 8.0
- Avidin-AMCA (Vector), S mg/ml in sterile distilled water, dilute 1:200
in PNM buffer.
Chemicals
and Supplies
Buffers and other
solutions