27 Yeast FISH: Delineation of Chromosomal Targets in Vegetative and Meiotic Yeast Cells
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Scheme 1. Flowchart of the preparation procedure for undisrupted (black arrowed route)
and spread nuclei (green and blue arrowed routes) for yeast FISH. Depending on the mode
of fixation prior to detergent spreading, either moderately spread (green arrows) or extensively spread nuclei (blue arrows) may be obtained. Nuclear spreads offer a higher resolution and thereby facilitate visual analysis of FISH signals. It should be noted that particular
three-dimensional chromosome arrangements are disrupted by extensive nuclear spreading
(see Fig. Id,e)