320
HARRY SCHERTHAN
11. DAPI banding patterns, which contribute to chromosome identification after FISH (for review see Carter 1994), may be contrast enhanced
by the addition of Actinomycin D (10 ~g/ml) to the DAPI containing
antifade solution. Alternatively, GTG banding prior to FISH may be
performed to identify chromosomes (Scherthan et al. 1989, 1994).
Although this approach is laborious, it may be followed when digital
image analysis is not feasible.
Acknowledgements. I am grateful to I.W. Gray, UCSF, for generously providing the linker
adaptor and pBS libraries, and to L. Fronicke, T. Raudsepp and B.P. Chowdhary for fruitful
collaborations.
References
Bouffler SD (1994) Whole-chromosome hybridization. Intn Rev Cytology 153;171-232
Carter NP (1994) Cytogenetic analysis by chromosome painting. Cytometry 18;2-10.
Comparative Genome Organization; First International Workshop on Comparative
Genome Organization. (1996) Mamm. Genome 7;717-734.
Collins C, Kuo WL, Segraves R, Pinkel D, Fuscoe J, Gray JW (1991) Construction and
characterization of plasmid libraries enriched in sequences from single human chromosomes. Genomics 11;997-1006.
Chowdhary BP, Fronicke L, Gustavsson I and Scherthan H (1996). Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping
based chromosomal homologies. Mamm Genome 7;297-302.
Chowdhary BP, Raudsepp T, Fronicke Land Scherthan H (1998) Emerging patterns of
comparative genome organization in some mammalian species as revealed by ZooFISH. Genome Research 8;577-589.
Dixkens C, Klett C, Bruch J, Kollak A, Serov OL, Zhdanova N, Vogel W, Hameister H
(1998) Zoo-FISH analysis in insectivores: "Evolution extols the virtue of the status
quo". Cytogenet Cell Genet 80;61-67.
Drouin R, Lemieux N, Richer CL (1990) Analysis of DNA replication during S-phase by
means of dynamic chromosome banding at high resolution. Chromosoma 99;273-80
Ferguson-Smith MA. (1997) Genetic analysis by chromosome sorting and painting:
phylogenetic and diagnostic applications. Em J Hum Genet.5;253-65.
Florijn RJ, Slats J, Tanke HI, and Raap AK (1995) Analysis of antifading reagents for
fluorescence microscopy. Cytometry 19;177-182.
Fronicke L (1997) Zoo-FISH-Untersuchungen zur Genomorganisation der Saugetiere.
PhD Thesis, FB Biologie, Univ. Kaiserslautern, Abt. Humanbiologie.
Fronicke L, Chowdhary BP, Scherthan Hand Gustavsson I (1996) A comparative map
of the porcine and human genomes demonstrates ZOO-FISH and gene mapping
based chromosomal homologies. Mamm Genome 7;285-290.
Fronicke Land Scherthan H (1997) Zoo-FISH analysis of human and Indian muntjac
karyotypes (Muntiacus muntjak vaginalis) reveals satellite-DNA clusters at the margins of conserved syntenic segments. Chromosome Res. 5;254-261.
HARRY SCHERTHAN
11. DAPI banding patterns, which contribute to chromosome identification after FISH (for review see Carter 1994), may be contrast enhanced
by the addition of Actinomycin D (10 ~g/ml) to the DAPI containing
antifade solution. Alternatively, GTG banding prior to FISH may be
performed to identify chromosomes (Scherthan et al. 1989, 1994).
Although this approach is laborious, it may be followed when digital
image analysis is not feasible.
Acknowledgements. I am grateful to I.W. Gray, UCSF, for generously providing the linker
adaptor and pBS libraries, and to L. Fronicke, T. Raudsepp and B.P. Chowdhary for fruitful
collaborations.
References
Bouffler SD (1994) Whole-chromosome hybridization. Intn Rev Cytology 153;171-232
Carter NP (1994) Cytogenetic analysis by chromosome painting. Cytometry 18;2-10.
Comparative Genome Organization; First International Workshop on Comparative
Genome Organization. (1996) Mamm. Genome 7;717-734.
Collins C, Kuo WL, Segraves R, Pinkel D, Fuscoe J, Gray JW (1991) Construction and
characterization of plasmid libraries enriched in sequences from single human chromosomes. Genomics 11;997-1006.
Chowdhary BP, Fronicke L, Gustavsson I and Scherthan H (1996). Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping
based chromosomal homologies. Mamm Genome 7;297-302.
Chowdhary BP, Raudsepp T, Fronicke Land Scherthan H (1998) Emerging patterns of
comparative genome organization in some mammalian species as revealed by ZooFISH. Genome Research 8;577-589.
Dixkens C, Klett C, Bruch J, Kollak A, Serov OL, Zhdanova N, Vogel W, Hameister H
(1998) Zoo-FISH analysis in insectivores: "Evolution extols the virtue of the status
quo". Cytogenet Cell Genet 80;61-67.
Drouin R, Lemieux N, Richer CL (1990) Analysis of DNA replication during S-phase by
means of dynamic chromosome banding at high resolution. Chromosoma 99;273-80
Ferguson-Smith MA. (1997) Genetic analysis by chromosome sorting and painting:
phylogenetic and diagnostic applications. Em J Hum Genet.5;253-65.
Florijn RJ, Slats J, Tanke HI, and Raap AK (1995) Analysis of antifading reagents for
fluorescence microscopy. Cytometry 19;177-182.
Fronicke L (1997) Zoo-FISH-Untersuchungen zur Genomorganisation der Saugetiere.
PhD Thesis, FB Biologie, Univ. Kaiserslautern, Abt. Humanbiologie.
Fronicke L, Chowdhary BP, Scherthan Hand Gustavsson I (1996) A comparative map
of the porcine and human genomes demonstrates ZOO-FISH and gene mapping
based chromosomal homologies. Mamm Genome 7;285-290.
Fronicke Land Scherthan H (1997) Zoo-FISH analysis of human and Indian muntjac
karyotypes (Muntiacus muntjak vaginalis) reveals satellite-DNA clusters at the margins of conserved syntenic segments. Chromosome Res. 5;254-261.