306
MARTIN LAWRIE, MARIA OCANA GIL, and DON CARDY
5. Blot with a piece of filter paper/tissue.
6. Leave for 10 min in the dark before viewing by epifluorescence microscopy using a Texas Red, FITC and DAPI triple band pass ftlter.
References
Knight S, Regan R, Nicod A, Horsley S, Kearney L, Homfray T, Winter R, Bolton P, Flint
J. Subtle Chromosome rearrangements in children with unexplained mental retardation. Lancet 1999. 354: 1676 - 1681
Flint J, Wilkie AOM, Buckle VJ, Winter RM, Holland AJ, McDermid HE: The detection
of subtelomeric chromosomal rearrangements in idiopathic mental retardation.
Nat. Genet. 1995. 9: 132- 139.
Giraudeau F, Aubert D, Young I, Horsley SW, Knight SJL, Kearney L, Vergnaud G, Flint
J: Molecular-cytogenetic detection of a deletion of Ip36.3. J. Med. Genet. 1997. 34:
314 -17
Knight S, Horsley S, Regan R, Lawrie M, Maher E, Cardy D, Flint J, Kearney 1. Development and clinical application of an innovative fluorescence in situ hybridisation
technique which detects submicroscopic rearrangements involving telomeres. Eur.
J. Hum. Genet. 1997 5: 1-8
European Polycystic Kidney Disease Consortium. Cell. 1994. 77:881-894
Lichter P, Cremer T, Tang C-JC, Watkins PC, Manuelidis L, Ward DC: Rapid detection
ofhuman chromosome 21 aberrations by in situ hybridization. Proc. Natl. Acad. Sci.
USA 1988. 85: 9138-9142.
Pinkel D, Landegent J, Collins C, Fuscoe J, Segraves R, Lucas J, Gray J: Fluorescence in
situ hybridization with human chromosome specific libraries: Detection of trisomy
21 and translocations of chromosome 4. Proc. Nat!. Acad. Sci .USA 1988.85: 96649668.
Collins C, Kuo WL, Segraves R, Fuscoe J, Pinkel D, Gray JW: Construction and characterization of plasmid libraries enriched in sequences from single human chromosomes. Genomics 199.1 11: 997-1006.
Schrock E, du Manoir S, Veldman T, Schoell B, Wienberg J, Ferguson-Smith MA, Ning
Y, Ledbetter DH, Bar-Am I, Soenksen D, Garini Y, Ried T: Multicolor spectral karyotyping of human chromosomes. Science 1996. 273:494-497
Speicher MR, Ballard SG, Ward DC: Karyotyping human chromosomes by combinatorial multifluor FISH. Nat. Genet. 1996.1 2:368-372
National Institutes of Health and Institute of Molecular Medicine Collaboration: A
complete set of human telomeric probes and their clinical application. Nat. Genet.
1996.14:86-89.
MARTIN LAWRIE, MARIA OCANA GIL, and DON CARDY
5. Blot with a piece of filter paper/tissue.
6. Leave for 10 min in the dark before viewing by epifluorescence microscopy using a Texas Red, FITC and DAPI triple band pass ftlter.
References
Knight S, Regan R, Nicod A, Horsley S, Kearney L, Homfray T, Winter R, Bolton P, Flint
J. Subtle Chromosome rearrangements in children with unexplained mental retardation. Lancet 1999. 354: 1676 - 1681
Flint J, Wilkie AOM, Buckle VJ, Winter RM, Holland AJ, McDermid HE: The detection
of subtelomeric chromosomal rearrangements in idiopathic mental retardation.
Nat. Genet. 1995. 9: 132- 139.
Giraudeau F, Aubert D, Young I, Horsley SW, Knight SJL, Kearney L, Vergnaud G, Flint
J: Molecular-cytogenetic detection of a deletion of Ip36.3. J. Med. Genet. 1997. 34:
314 -17
Knight S, Horsley S, Regan R, Lawrie M, Maher E, Cardy D, Flint J, Kearney 1. Development and clinical application of an innovative fluorescence in situ hybridisation
technique which detects submicroscopic rearrangements involving telomeres. Eur.
J. Hum. Genet. 1997 5: 1-8
European Polycystic Kidney Disease Consortium. Cell. 1994. 77:881-894
Lichter P, Cremer T, Tang C-JC, Watkins PC, Manuelidis L, Ward DC: Rapid detection
ofhuman chromosome 21 aberrations by in situ hybridization. Proc. Natl. Acad. Sci.
USA 1988. 85: 9138-9142.
Pinkel D, Landegent J, Collins C, Fuscoe J, Segraves R, Lucas J, Gray J: Fluorescence in
situ hybridization with human chromosome specific libraries: Detection of trisomy
21 and translocations of chromosome 4. Proc. Nat!. Acad. Sci .USA 1988.85: 96649668.
Collins C, Kuo WL, Segraves R, Fuscoe J, Pinkel D, Gray JW: Construction and characterization of plasmid libraries enriched in sequences from single human chromosomes. Genomics 199.1 11: 997-1006.
Schrock E, du Manoir S, Veldman T, Schoell B, Wienberg J, Ferguson-Smith MA, Ning
Y, Ledbetter DH, Bar-Am I, Soenksen D, Garini Y, Ried T: Multicolor spectral karyotyping of human chromosomes. Science 1996. 273:494-497
Speicher MR, Ballard SG, Ward DC: Karyotyping human chromosomes by combinatorial multifluor FISH. Nat. Genet. 1996.1 2:368-372
National Institutes of Health and Institute of Molecular Medicine Collaboration: A
complete set of human telomeric probes and their clinical application. Nat. Genet.
1996.14:86-89.