21 The Combined Use of Nuclease in Situ Digestion and FISH (NU-FISH)
281
References
Burkholder GD (1989) Morphological and biochemical effects of endonucleases on
isolated mammalian chromosomes in vitro. Chromosoma 97:347-355
Fernandez JL, Campos A, Lopez-Fernandez C, Gosalvez J, Goyanes V (1995) Difference
in constitutive heterochromatin behaviour between human amniocytes and lymphocytes detected by a sequential "in situ" exonuclease III digestion-random primer
extension procedure. J Med Genet 32:32-35
Fernandez JL, Goyanes V, Ramiro-Diez J, Gosalvez J (1998) Evidence of a differential
organization of chromatid containing terminal or interstitial (TTAGGG)n repeats
by in situ digestion with nucleases. Cytogenet Cell Genet 82:195-198
Fernandez JL, Valverde D, Goyanes V, Buno I, Gosalvez J (1997) AluI in situ digestion
of human alphoid and classical satellite DNA regions. High-resolution digital image
analysis of FISH signal from condensed and extended chromatin. Cytogenet Cell
Genet 76:94-100
GosaIvez J, Lopez-Fernandez C, Fernandez JL, Goyanes V (1993) In situ oligonucleotide priming to detect induced DNA breaks under electron microscopy. TIG 9:156157
Gosalvez J, Lopez-Fernandez C, Goyanes V, Mezzanotte R (l997a) Chromosome differentiation using nucleases. An overview. In: Henriques-Gil N, Parker JS, Puertas
MJ (eds) Chromosomes Today, vol. 12. Chapman and Hall, London, pp 23-49
GosaIvez J, Mason PL, Lopez-Fernandez C (l997b) Differentiation of individuals, populations and species of Orthoptera: the past, present and future of chromosome
markers. In: Gangwere SK, Muralirangan MC, Meera Muralirangan (eds) The Bionomics of Grasshoppers, Katydids and their Kin. CAB International. University
Press, Cambridge, pp 355-383
Lopez-Fernandez C, Gosalvez J, Ferrucci L, Mezzanotte R (1991) Restriction endonucleases in the study of eukaryotic chromosomes. Genetica 83:257-274
Mezzanotte R, Manconni PE, Rotzzo C, Ennas MG, Ferrucci L (1985) The banding
pattern produced by restriction endonucleases. Bas Appl Histochem 29:115-120
Torre J de la, Sumner AT (1994) Analysis of chromosomes with Restriction Endonucleases and DNase hypersensitivity. In: Gosden JR (ed) Chromosome Analysis Protocols. Methods in Molecular Biology, vol. 29. Humana Press, New Jersey, pp 123139.
human lymphocyte chromosomes, yielding a low hybridisation signal. Bal31 shows a high
enzymatic activity; removes a considerable amount ofdsDNA in the target area, so after heat
denaturation, a faint FISH signal is evident compared with that of its control. g-i Methylation
detection after NU-FISH on the B-chromosomes ofEyprepocnemis plorans. g Control chromosomes (without previous RE-digestion) after FISH using an rDNA probe. h-i B-chromosomes digested with MspI (h) and with Hpall (i) and probed with rDNA. Note the reduction of
the fluorescence intensity only on the B-chromosomes digested with MspI. (All images except
g-i with permission of S. Karger AG)
281
References
Burkholder GD (1989) Morphological and biochemical effects of endonucleases on
isolated mammalian chromosomes in vitro. Chromosoma 97:347-355
Fernandez JL, Campos A, Lopez-Fernandez C, Gosalvez J, Goyanes V (1995) Difference
in constitutive heterochromatin behaviour between human amniocytes and lymphocytes detected by a sequential "in situ" exonuclease III digestion-random primer
extension procedure. J Med Genet 32:32-35
Fernandez JL, Goyanes V, Ramiro-Diez J, Gosalvez J (1998) Evidence of a differential
organization of chromatid containing terminal or interstitial (TTAGGG)n repeats
by in situ digestion with nucleases. Cytogenet Cell Genet 82:195-198
Fernandez JL, Valverde D, Goyanes V, Buno I, Gosalvez J (1997) AluI in situ digestion
of human alphoid and classical satellite DNA regions. High-resolution digital image
analysis of FISH signal from condensed and extended chromatin. Cytogenet Cell
Genet 76:94-100
GosaIvez J, Lopez-Fernandez C, Fernandez JL, Goyanes V (1993) In situ oligonucleotide priming to detect induced DNA breaks under electron microscopy. TIG 9:156157
Gosalvez J, Lopez-Fernandez C, Goyanes V, Mezzanotte R (l997a) Chromosome differentiation using nucleases. An overview. In: Henriques-Gil N, Parker JS, Puertas
MJ (eds) Chromosomes Today, vol. 12. Chapman and Hall, London, pp 23-49
GosaIvez J, Mason PL, Lopez-Fernandez C (l997b) Differentiation of individuals, populations and species of Orthoptera: the past, present and future of chromosome
markers. In: Gangwere SK, Muralirangan MC, Meera Muralirangan (eds) The Bionomics of Grasshoppers, Katydids and their Kin. CAB International. University
Press, Cambridge, pp 355-383
Lopez-Fernandez C, Gosalvez J, Ferrucci L, Mezzanotte R (1991) Restriction endonucleases in the study of eukaryotic chromosomes. Genetica 83:257-274
Mezzanotte R, Manconni PE, Rotzzo C, Ennas MG, Ferrucci L (1985) The banding
pattern produced by restriction endonucleases. Bas Appl Histochem 29:115-120
Torre J de la, Sumner AT (1994) Analysis of chromosomes with Restriction Endonucleases and DNase hypersensitivity. In: Gosden JR (ed) Chromosome Analysis Protocols. Methods in Molecular Biology, vol. 29. Humana Press, New Jersey, pp 123139.
human lymphocyte chromosomes, yielding a low hybridisation signal. Bal31 shows a high
enzymatic activity; removes a considerable amount ofdsDNA in the target area, so after heat
denaturation, a faint FISH signal is evident compared with that of its control. g-i Methylation
detection after NU-FISH on the B-chromosomes ofEyprepocnemis plorans. g Control chromosomes (without previous RE-digestion) after FISH using an rDNA probe. h-i B-chromosomes digested with MspI (h) and with Hpall (i) and probed with rDNA. Note the reduction of
the fluorescence intensity only on the B-chromosomes digested with MspI. (All images except
g-i with permission of S. Karger AG)