20 Strand-Specific Fluorescence in Situ Hybridization: CO-FISH and COD-FISH
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Fig. 2. Examples of CO-FISH and COD-FISH on human and mouse chromosomes. The
hybridization sites appearing as white areas indicate probes labeled with FITC, while probes
labeled with Texas Red have been pseudocolored black. a Human chromosome-l hybridized with repetitive sequence probe specific for the long arm heterochromatic region.
Chromosome on left is from a slide hybridized with only one strand of the sequence. Chromosome on the right was sequentially hybridized with probes from both strands of the
sequence, one strand was labeled with FITC and the other with Texas red. b Human chromosome-5 hybridized with the telomere repeat probe. On the left is a chromosome after
hybridization with the C-rich strand. On the right is the same chromosome after a second,
sequential labeling with the G-rich strand. c Homologues of human chromosome-X hybridized with both the C-rich strand of the telomere sequence and a single-stranded probe of
an alpha-satellite DNA sequence specific for the centromeric region of chromosome-X.
d Homologues of human chromosome-8 hybridized with both the C-rich strand of the telomere sequence and a single-stranded probe of an alpha-satellite DNA sequence specific for
the centromeric region of chromosome-8. The change in the positions of the telomere hybridization sites relative to the centromeric site indicate the presence of a pericentromeric
inversion. e Mouse chromosomes hybridized with a single-stranded probe of the mouse
major satellite. f Mouse chromosomes hybridized with a single-stranded probe of the telomere repeat
269
Fig. 2. Examples of CO-FISH and COD-FISH on human and mouse chromosomes. The
hybridization sites appearing as white areas indicate probes labeled with FITC, while probes
labeled with Texas Red have been pseudocolored black. a Human chromosome-l hybridized with repetitive sequence probe specific for the long arm heterochromatic region.
Chromosome on left is from a slide hybridized with only one strand of the sequence. Chromosome on the right was sequentially hybridized with probes from both strands of the
sequence, one strand was labeled with FITC and the other with Texas red. b Human chromosome-5 hybridized with the telomere repeat probe. On the left is a chromosome after
hybridization with the C-rich strand. On the right is the same chromosome after a second,
sequential labeling with the G-rich strand. c Homologues of human chromosome-X hybridized with both the C-rich strand of the telomere sequence and a single-stranded probe of
an alpha-satellite DNA sequence specific for the centromeric region of chromosome-X.
d Homologues of human chromosome-8 hybridized with both the C-rich strand of the telomere sequence and a single-stranded probe of an alpha-satellite DNA sequence specific for
the centromeric region of chromosome-8. The change in the positions of the telomere hybridization sites relative to the centromeric site indicate the presence of a pericentromeric
inversion. e Mouse chromosomes hybridized with a single-stranded probe of the mouse
major satellite. f Mouse chromosomes hybridized with a single-stranded probe of the telomere repeat