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HEINZ-ULRICH G. WEIER
- 4, 6-diamino-2-phenylindole (DAPI) (Calbiochem), 0.05 Ilg/ml in antifade solution. Store at -20°C.
- dATP, dCTP, dGTP, dTTP: 100 mM each (Roche or Pharmacia). Store
at -20°C.
- Digoxigenin-ll-dUTP: 1 mM (Roche Molecular Biochemicals). Store at
-20°C.
- lOx dNTP mix: dATP, dCTP, dGTP, and dTTP, 10 mM each.
- EDTA (ethylenediamine tetraacetic acid): 0.5 M (pH 8.0) (Life Technologies).
- Ethidium bromide, 10 mg/ml (Life Technologies)
- Fluorescein avidin DCS (avidin-FITC, Vector), dilute stock solution
(2 mg/ml in PNM) 1:100 with PNM. Store at 4°C.
- Fluorescein-12-dUTP, 1 mM (Roche Molecular Biochemicals). Store at
-20°C.
- Formamide (FA, Life Technologies or Roche). Store at 4°C.
- Glycogen, 20 mg/ml (Roche Molecular Biochemicals). Store at -20°C.
- Geneclean II kit (BIO 101)
- Human con DNA, 1 mg/ml (Life Technologies). Store at -20°C.
- Lambda phage DNA (Roche or Life Technologies). Prepare 2.5 ng/1l1 in
2xSSC. Store at 4°C.
- Low melting point agarose (Biorad)
- Lysozyme (Sigma Chemicals): prepare stock (50 mg/ml in 10 mM Tris,
pH 7.5), and store in aliquots at -20°C. Do not refreeze.
- lOx PCR buffer: 15 mM MgCh, 100mM TrisHCI, 500 mM KCI, 0.01%
gelatin.
- Phenol/Chloroform/Isoamyl alcohol: 25/24/1(vol.lvol.lvol.)(Life Technologies). Store at 4°C.
- Random-Priming Kit: BioPrime kit (Life Technologies). Store at -20°C.
- Proteinase K (Roche Molecular Biochemicals): 20 mg/ml in 10 mM
TrisHCI, pH 7.5. Store at -20°C.