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BURT G. FEUERSTEIN et a1.
limits of normal. It then remains to quantify how the fluorescence ratio of
DNA extracted from genetically abnormal tissue compares with that extracted from the normal tissue. A statistical treatment of this method has
been published (16).
Conclusions
Comparative genomic hybridization scans the genome of an organism for
relative changes in copy number. DNA from fresh frozen or from fixed and
paraffin embedded tissue can be utilized. DNA preparation, labeling, metaphase preparation, hybridization, and analysis are important steps for a
successful experiment. Expert and knowledgeable support for these procedures is important to obtain reproducible and accurate results.
References
du Manoir, S., Speicher, M. R., Joos, S., Schrock, E., Popp, S., Dohner, H., Kovacs, G.,
Robert-Nicoud, M., Lichter, P., and Cremer, T. Detection of complete and partial
chromosome gains and losses by comparative genomic in situ hybridization, Hum
Genet. 90: 590-610, 1993.
Kallioniemi, A., Kallioniemi, O. P., Sudar, D., Rutovitz, D., Gray, J. W., Waldman, F.,
and Pinkel, D. Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors, Science. 258: 818-21, 1992.
Kallioniemi, O. P., Kallioniemi, A., Sudar, D., Rutovitz, D., Gray, J. W., Waldman, F.,
and Pinkel, D. Comparative genomic hybridization: a rapid new method for detecting and mapping DNA amplification in tumors, Semin Cancer BioI. 4: 41-6, 1993.
Mohapatra, G., Kim, D. H., and Feuerstein, B. G. Detection of multiple gains and losses
of genetic material in ten glioma cell lines by comparative genomic hybridization,
Genes Chromosomes Cancer. 13: 86-93, 1995.
Kuukasjarvi, T., Tanner, M., Pennanen, S., Karhu, R., Visakorpi, T., and Isola, J. Optimizing DOP-PCR for universal amplification of small DNA samples in comparative
genomic hybridization, Genes Chromosomes Cancer. 18: 94-101, 1997.
Telenius, H., Carter, N. P., Bebb, C. E., Nordenskjold, M., Ponder, B. A., and Tunnacliffe, A. Degenerate oligonucleotide-primed PCR: general amplification of target
DNA by a single degenerate primer, Genomics. 13: 718-25, 1992.
Sanchez-Cespedes, M., Cairns, P., Jen, J., and Sidransky, D. Degenerate oligonucleotide-primed PCR (DOP-PCR): evaluation of its reliability for screening of genetic
alterations in neoplasia, Biotechniques. 25: 1036-8, 1998.
Spurbeck, J. L., Zinsmeister, A. R., Meyer, K. J., and Jalal, S. M. Dynamics of chromosome spreading, Am J Med Genet. 61: 387-93, 1996.
Dracopoli, N. C. Cytogenetics. Current protocols in human genetics, pp. 2 v. New York,
NY: John Wiley & Sons, 1994.
BURT G. FEUERSTEIN et a1.
limits of normal. It then remains to quantify how the fluorescence ratio of
DNA extracted from genetically abnormal tissue compares with that extracted from the normal tissue. A statistical treatment of this method has
been published (16).
Conclusions
Comparative genomic hybridization scans the genome of an organism for
relative changes in copy number. DNA from fresh frozen or from fixed and
paraffin embedded tissue can be utilized. DNA preparation, labeling, metaphase preparation, hybridization, and analysis are important steps for a
successful experiment. Expert and knowledgeable support for these procedures is important to obtain reproducible and accurate results.
References
du Manoir, S., Speicher, M. R., Joos, S., Schrock, E., Popp, S., Dohner, H., Kovacs, G.,
Robert-Nicoud, M., Lichter, P., and Cremer, T. Detection of complete and partial
chromosome gains and losses by comparative genomic in situ hybridization, Hum
Genet. 90: 590-610, 1993.
Kallioniemi, A., Kallioniemi, O. P., Sudar, D., Rutovitz, D., Gray, J. W., Waldman, F.,
and Pinkel, D. Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors, Science. 258: 818-21, 1992.
Kallioniemi, O. P., Kallioniemi, A., Sudar, D., Rutovitz, D., Gray, J. W., Waldman, F.,
and Pinkel, D. Comparative genomic hybridization: a rapid new method for detecting and mapping DNA amplification in tumors, Semin Cancer BioI. 4: 41-6, 1993.
Mohapatra, G., Kim, D. H., and Feuerstein, B. G. Detection of multiple gains and losses
of genetic material in ten glioma cell lines by comparative genomic hybridization,
Genes Chromosomes Cancer. 13: 86-93, 1995.
Kuukasjarvi, T., Tanner, M., Pennanen, S., Karhu, R., Visakorpi, T., and Isola, J. Optimizing DOP-PCR for universal amplification of small DNA samples in comparative
genomic hybridization, Genes Chromosomes Cancer. 18: 94-101, 1997.
Telenius, H., Carter, N. P., Bebb, C. E., Nordenskjold, M., Ponder, B. A., and Tunnacliffe, A. Degenerate oligonucleotide-primed PCR: general amplification of target
DNA by a single degenerate primer, Genomics. 13: 718-25, 1992.
Sanchez-Cespedes, M., Cairns, P., Jen, J., and Sidransky, D. Degenerate oligonucleotide-primed PCR (DOP-PCR): evaluation of its reliability for screening of genetic
alterations in neoplasia, Biotechniques. 25: 1036-8, 1998.
Spurbeck, J. L., Zinsmeister, A. R., Meyer, K. J., and Jalal, S. M. Dynamics of chromosome spreading, Am J Med Genet. 61: 387-93, 1996.
Dracopoli, N. C. Cytogenetics. Current protocols in human genetics, pp. 2 v. New York,
NY: John Wiley & Sons, 1994.