16 Comparative Genomic Hybridization
199
-
-
ORMAL
D A
-
- -
-CoT-}
D A
~:lt~?::~::.:;~::~::·;:·~:·~::.·,::=:::·:~ '\:'?~/',' ~
.:: .. , " , ';: ',. : .. ,'.; .
:'~'::"::':':":.:'. ' ; ::. ,', ,"::..:: ": :; .
;~::·:;:ii~~i~:·~:{}:~~:::::\:;:;:· .~ ;~::. ;i ; L.::
-- -
-
- -
-
- -
~/
-
-
- -
TEST
D A
---~.
/
c
A
B
G
~-....,l.t-J.~~~
----l~_D
Intensity
I
E
Copy
l
umber
Ratio
Fig. I. Comparative Genomic Hybridization. A Test DNA, here tagged in green, reference
DNA, here tagged in red, and unlabeled CoT-1 DNA are hybridized to B normal metaphase
chromosomes. C The tagged sequences will produce signals at homologous sites on the
metaphase chromosome. D If the intensity of test sequence (green line) measured at a location is less than the intensity of the reference sequence (red line) measured there, E the copy
number ratio is less than one, and there is a loss (L) ofgenetic material at that location. If the
intensity of test sequence measured at a location is greater than the intensity of the reference
sequence measured there, the copy number ratio is more than one, and there is gain or
amplification (G)