8 Amniotic Fluid Cells - Uncultured
119
- Coplin jars
- precleaned microscope slides
- glass coverslips (20x20 mm, 15x 15 ffiffi, 24x60 mm)
- polypropylene microcentrifuge tubes (0.5 or 1.5 ml)
- centrifuge tube 10-15 ml (conical)
- graduated cylinder
- humidified chamber
- rubber cement
- Ethanol (100%)
- concentrated HCI (12 M)
- NaOH (1 M)
- purified water
- Carnoy's fixative (3:1 methanol/acetic acid)
- MgCh, sodium citrate, NaCI
- 10 x PBS (phosphate buffered saline)
- 20xSSC (salt sodium citrate)
- pepsin (from hog stomach, Fluka; prepare a stock solution of 10%
pepsin)
- Hyaluronidase (from bovine testes, Sigma; prepare a stock solution
of ca. 50 IU per ml)
- Dabco (Triethylenediamine, by Sigma)
- Vectashield mounting medium (Vector Laboratories)
- DAPI (4,6-diamidino-2-phenylindol, prepare a stock solution of
D.1 mg/IOD ml)
- NP 4D (included in the Vysis-kit)
- Paraformaldehyde (37%)
Reagents
and buffers