114
THOMAS LIEHR
4. Incubation can be stopped - if necessary - after 48 or 96 h, as well.
While in the first case weaker signals are possible, in the second
case some cross-hybridization problems may arise.
5. During the washing steps it is important to prevent the slide surfaces
drying out, otherwise background problems may arise.
Acknowledgements. Supported by the EU (ICA2-CT-2000-10012 and QLRT-1999-31590).
The continuos support of the Carl Zeiss GmbH (Jena, Germany) is gratefully acknowledged.
References
Lampel, S, Steilen, H, Zang, KD, Wullich, B (1993) Sex chromatin in hair roots-25 years
later: fluorescence in situ hybridization of hair root cells for detection of numerical
chromosome aberrations. Cytogenet Cell Genet, 63:244-246
Liehr, T, Thoma, K, Kammler, K, Gehring, C, Ekici, A, Bathke, KD, Grehl, H, Rautenstrauss, B (1995) Direct preparation of uncultured EDTA-treated or heparinized
blood for interphase FISH analysis. Appl Cytogenet, 21:185-188
Liehr, T, Rautenstrauss, B, Grehl, H, Bathke, KD, Ekici, A, Rauch, A, Rott, HD (1996)
Mosaicism for the Charcot-Marie-Tooth disease type lA duplication suggests somatic reversion. Human Genet, 98:22-28
Pfeiffer, RA, Schulze, T (1994) Mosaicism in three cases 47,XY (or XX), +i(l8)(pl0)
detected by interphase FISH of buccal mucosa. Ann Genet 37:210-214
Rothenmund, H, Chudley, AE, Dawson, AJ (1997) Familial transmission of a small
supernumerary marker chromosome 8 identified by FISH: an update. Am J Med
Genet, 72:339-334
THOMAS LIEHR
4. Incubation can be stopped - if necessary - after 48 or 96 h, as well.
While in the first case weaker signals are possible, in the second
case some cross-hybridization problems may arise.
5. During the washing steps it is important to prevent the slide surfaces
drying out, otherwise background problems may arise.
Acknowledgements. Supported by the EU (ICA2-CT-2000-10012 and QLRT-1999-31590).
The continuos support of the Carl Zeiss GmbH (Jena, Germany) is gratefully acknowledged.
References
Lampel, S, Steilen, H, Zang, KD, Wullich, B (1993) Sex chromatin in hair roots-25 years
later: fluorescence in situ hybridization of hair root cells for detection of numerical
chromosome aberrations. Cytogenet Cell Genet, 63:244-246
Liehr, T, Thoma, K, Kammler, K, Gehring, C, Ekici, A, Bathke, KD, Grehl, H, Rautenstrauss, B (1995) Direct preparation of uncultured EDTA-treated or heparinized
blood for interphase FISH analysis. Appl Cytogenet, 21:185-188
Liehr, T, Rautenstrauss, B, Grehl, H, Bathke, KD, Ekici, A, Rauch, A, Rott, HD (1996)
Mosaicism for the Charcot-Marie-Tooth disease type lA duplication suggests somatic reversion. Human Genet, 98:22-28
Pfeiffer, RA, Schulze, T (1994) Mosaicism in three cases 47,XY (or XX), +i(l8)(pl0)
detected by interphase FISH of buccal mucosa. Ann Genet 37:210-214
Rothenmund, H, Chudley, AE, Dawson, AJ (1997) Familial transmission of a small
supernumerary marker chromosome 8 identified by FISH: an update. Am J Med
Genet, 72:339-334