Atrial Natriuretic Peptides in Antarctic Fish
lO r
35 j
I
3) T
25 1
217
• T","~Ir>m
I 0 CINc>rtochco" I
Fig. 2. ANP-like immunoreactivity in heart homogenates and plasma from C. hamatus and
T bernacchii. Values are expressed as pglmg protein in heart homogenates and pg/O. I ml in
plasma (means ± SO). The difference between the two species is statistically significant
(Student's t-test, p<0.05)
It is possible that the synthesis is more active in the icefish than in other
fish. In the hemoglobin-free fish, blood volume and cardiac output are
several fold greater than in the red-blooded species, to compensate for'the
reduced oxygen-carrying capacity of the blood [23]. SDS-PAGE of heart
homogenates from Champsocephalus gunnari [24] revealed a band whose
electrophoretic mobility resembled that ofrANP. Western immunoblotting,
using the rabbit antisera to rANP, showed that the band at that position was
conjugated with the corresponding antibody (Fig. 3).
WB
ANP
s
Fig. 3. SOS-PAGE and Western immunoblotting of crude extract from the heart of C.
gunnari. Lane WB : Western blot with antibodies to ANP showing a band at about 3.0 kDa;
Lane ANP: SOS-Page of standard ANP stained with Silver-staining; Lane S: separation of
heart proteins by l5%T gradient SOS-PAGE, stained with Silver-staining
lO r
35 j
I
3) T
25 1
217
• T","~Ir>m
I 0 CINc>rtochco" I
Fig. 2. ANP-like immunoreactivity in heart homogenates and plasma from C. hamatus and
T bernacchii. Values are expressed as pglmg protein in heart homogenates and pg/O. I ml in
plasma (means ± SO). The difference between the two species is statistically significant
(Student's t-test, p<0.05)
It is possible that the synthesis is more active in the icefish than in other
fish. In the hemoglobin-free fish, blood volume and cardiac output are
several fold greater than in the red-blooded species, to compensate for'the
reduced oxygen-carrying capacity of the blood [23]. SDS-PAGE of heart
homogenates from Champsocephalus gunnari [24] revealed a band whose
electrophoretic mobility resembled that ofrANP. Western immunoblotting,
using the rabbit antisera to rANP, showed that the band at that position was
conjugated with the corresponding antibody (Fig. 3).
WB
ANP
s
Fig. 3. SOS-PAGE and Western immunoblotting of crude extract from the heart of C.
gunnari. Lane WB : Western blot with antibodies to ANP showing a band at about 3.0 kDa;
Lane ANP: SOS-Page of standard ANP stained with Silver-staining; Lane S: separation of
heart proteins by l5%T gradient SOS-PAGE, stained with Silver-staining