Chapter 19
Accumulation of Okadaic Acid and Detoxifying
Enzymes in the Digestive Gland of Mytilus
galloprovincialis During Exposure to DSP
A. Vidal, Y. Ruiz, P. Su´ arez, Ana Alonso Martinez, A.E. Rossignoli, J. Blanco,
O. Garcia, and F. San Juan
Introduction
In the Galician Rias, Dinophysis acuminata and Dinophysis acuta are the main
species of toxic microalgae, and have been shown to produce okadaic acid
and dinophysistoxin-2 (Fern´ andez et al. 1998). Okadaic acid, diniphysistoxin-1
(DTX-1) and dinophysistoxin-2 (DTX-2) have been identified in most Diarrhetic
Shellfish Poisoning (DSP) episodes in Galician mussels (Gago-Martinez et al.
1996), as well as being found in Irish (Carmody et al. 1996) and Portuguese (Vale
and Sampayo 2002) shellfish.
OA and Dinophysistoxins (DTX’s) are the principal toxic compounds causing
DSP in humans (Yasumoto et al. 1985). These compounds are lipophilic polyether
molecules produced by dinoflagellates Dinophysis sp. and Prorocentrum sp. genera
(Yasumoto et al. 1978; Murata et al. 1982).
A. Vidal • Y. Ruiz • P. Su´ arez • F. San Juan ()
Department of Biochemistry, Genetics and Immunology, University of Vigo, Vigo, Spain
e-mail: adrianavidal@uvigo.es; yruiz@uvigo.es; psuarez@uvigo.es; fsanjuan@uvigo.es
A.E. Rossignoli • J. Blanco
Centro de Investigaci´ ons Mari˜ nas (Xunta de Galicia), Pontevedra, Spain
e-mail: ara@cimacoron.org; juan.blanco@cimacoron.org
O. Garcia
Department of Biochemistry and Molecular Biology, University of Santiago de Compostela,
Santiago de Compostela, Spain
A. Alonso Martinez
Department of Biochemistry, Genetics and Immunology, Faculty of Sciences,
University of Vigo, Lagoas-Marcosende s/n, 36310 Vigo, Spain
e-mail: amam@uvigo.es
G. Sauv´ e (ed.), Molluscan Shellfish Safety, DOI 10.1007/978-94-007-6588-7 19,
© Springer ScienceCBusiness Media Dordrecht 2014
217
Accumulation of Okadaic Acid and Detoxifying
Enzymes in the Digestive Gland of Mytilus
galloprovincialis During Exposure to DSP
A. Vidal, Y. Ruiz, P. Su´ arez, Ana Alonso Martinez, A.E. Rossignoli, J. Blanco,
O. Garcia, and F. San Juan
Introduction
In the Galician Rias, Dinophysis acuminata and Dinophysis acuta are the main
species of toxic microalgae, and have been shown to produce okadaic acid
and dinophysistoxin-2 (Fern´ andez et al. 1998). Okadaic acid, diniphysistoxin-1
(DTX-1) and dinophysistoxin-2 (DTX-2) have been identified in most Diarrhetic
Shellfish Poisoning (DSP) episodes in Galician mussels (Gago-Martinez et al.
1996), as well as being found in Irish (Carmody et al. 1996) and Portuguese (Vale
and Sampayo 2002) shellfish.
OA and Dinophysistoxins (DTX’s) are the principal toxic compounds causing
DSP in humans (Yasumoto et al. 1985). These compounds are lipophilic polyether
molecules produced by dinoflagellates Dinophysis sp. and Prorocentrum sp. genera
(Yasumoto et al. 1978; Murata et al. 1982).
A. Vidal • Y. Ruiz • P. Su´ arez • F. San Juan ()
Department of Biochemistry, Genetics and Immunology, University of Vigo, Vigo, Spain
e-mail: adrianavidal@uvigo.es; yruiz@uvigo.es; psuarez@uvigo.es; fsanjuan@uvigo.es
A.E. Rossignoli • J. Blanco
Centro de Investigaci´ ons Mari˜ nas (Xunta de Galicia), Pontevedra, Spain
e-mail: ara@cimacoron.org; juan.blanco@cimacoron.org
O. Garcia
Department of Biochemistry and Molecular Biology, University of Santiago de Compostela,
Santiago de Compostela, Spain
A. Alonso Martinez
Department of Biochemistry, Genetics and Immunology, Faculty of Sciences,
University of Vigo, Lagoas-Marcosende s/n, 36310 Vigo, Spain
e-mail: amam@uvigo.es
G. Sauv´ e (ed.), Molluscan Shellfish Safety, DOI 10.1007/978-94-007-6588-7 19,
© Springer ScienceCBusiness Media Dordrecht 2014
217