70
Methods for Setting Up Primary Cultures Specific to Animal Groups
Results
Results similar to those of primary cultures from embryonic tissues will
be obtained (Fig. 23). Several cell lines have been established using this
method (Sohi, 1973; Sato, 1989; Hara et aI., 1993).
• References
Carlson, J.G. (1946) Protoplasmic viscosity changes in different regions of the grasshopper neuroblast during mitosis. BioI. Bull. 90,109-121.
Chao, J. and Ball, G.H. (1971) A cell line isolated from hemocytes of Samia cynthia pupae. Curro Top.
Microbiol. Immunol. 55, 28-32.
Eide, P.E., Coldwell, J.M. and Marks, E.P. (1975) Establishment of two cell lines from embryonic
tissue of the tobacco hornworm, Manduca sexta (1.). In Vitro 11,395-399.
Grace, T.D.C. (1962) Establishment of four strains of cells from insect tissues grown in vitro. Nature
195,788-789.
Grace, T.D.C. (1967) Establishment of a line of cells from the silkworm, Bombyx mori. Nature 216,
613.
Hara, K., Funakoshi, M., Tsuda, K. and Kawarabata, T. (1993) New Spodoptera exigua cell lines susceptible to Spodoptera exigua nuclear polyhedrosis virus. In Vitro Cell. Dev. BioI. 29A, 904907.
Hink, W.P. (1970) Established insect cell line from Trichoplusia ni. Nature 226,466-467.
Hink, W.P. and Ellis, B.J. (1971) Establishment and characterization of two new cell lines (CP-1268
and CP-169) from the codling moth, Carpocapsa pomonella. Curro Top. Microbiol. Immunol.
55,19-28.
Hink, W.P. and Ignoffo, C.M. (1970) Establishment of a new cell line (IMC-HZ-l) from ovaries of
cotton ballworm moth, Heliothis zea (boddie). Exp. Cell Res. 60, 307-309.
Inoue, H. and Mitsuhashi, J. (1984) A Bombyx mori cell line susceptible to a nuclear polyhedrosis
virus. J. Sericul. Sci. Jpn. 53,108-113.
Loeb, M.J. and Hakim, R.S. (1996) Insect midgut epithelium in vitro: an insect stem cell system. J.
Insect Physiol. 42, 11 03-1111.
Loeb, M.J., Jaffe, H., Gelman, D.B. and Hakim, R.S. (1999) Two polypeptide factors that promote
differentiation of insect midgut stem cells in vitro. Arch. Insect Biochem. Physiol. 40, 129-140.
Lynn, D.E., Miller, S.G. and Oberlander, H. (1982) Establishment of a cell line from lepidopteran
wing imaginal discs: induction of newly synthesized proteins by 20-hydroxyecdysone. Proc.
Nat! Acad. Sci. USA, BioI. Sci. 79,2589-2593.
Lynn, D.E. and Oberlander, H. (1983) The establishment of cell lines from imaginal wing discs of
Spodoptera frugiperda and Plodia interpunctella. J. Insect Physiol. 29, 591-596.
Lynn, D.E. and Oberlander, H. (1986) Obtainment of hormonally sensitive cell lines from imaginal
discs of Lepidoptera species. In: Kurstak, E (ed.) Techniques in the Life Sciences, Vol. C-2,
Techniques in in vitro invertebrate hormones and genes, C213, Elsevier Sci. Pub., County Clare,
1-12.
Mano, C. and Mitsuhashi, J. (1981)Primary culture of various tissues in the greater wax moth, Galleria mellonella (Insecta, Lepidoptera). Zool. Mag. 90, 76-84 (in Japanese with an English abstract).
Miltenburger, H.G., David, P., Mahr, U. and Zipp, W. (1977) Uber die Erstellung von LepidopterenDauerzellinien und die in vitro-Replikation von insektenpathogenen Vieren. I. Mamestra
brassicae 1. (Kohleule)- Zellinien und NPV-Replikation. Z. P. Ang. Entomol. 82,306-323.
Mitsuhashi, J. (1965) Tissue culture of the rice stem borer, Chilo suppressalis Walker (Lepidoptera:
Pyralidae). I. Cell migration from the explanted tissues of diapausing larvae. Jpn. J. App!.
Entomo!' Zool. 9, 137-141.
Mitsuhashi, J. (1972) Primary culture of hemocytopoietic tissue of Papilio xuthus Linne (Lepidoptera: Papilionidae). App!. Entomo!' Zoo!. 7, 39-41.
Methods for Setting Up Primary Cultures Specific to Animal Groups
Results
Results similar to those of primary cultures from embryonic tissues will
be obtained (Fig. 23). Several cell lines have been established using this
method (Sohi, 1973; Sato, 1989; Hara et aI., 1993).
• References
Carlson, J.G. (1946) Protoplasmic viscosity changes in different regions of the grasshopper neuroblast during mitosis. BioI. Bull. 90,109-121.
Chao, J. and Ball, G.H. (1971) A cell line isolated from hemocytes of Samia cynthia pupae. Curro Top.
Microbiol. Immunol. 55, 28-32.
Eide, P.E., Coldwell, J.M. and Marks, E.P. (1975) Establishment of two cell lines from embryonic
tissue of the tobacco hornworm, Manduca sexta (1.). In Vitro 11,395-399.
Grace, T.D.C. (1962) Establishment of four strains of cells from insect tissues grown in vitro. Nature
195,788-789.
Grace, T.D.C. (1967) Establishment of a line of cells from the silkworm, Bombyx mori. Nature 216,
613.
Hara, K., Funakoshi, M., Tsuda, K. and Kawarabata, T. (1993) New Spodoptera exigua cell lines susceptible to Spodoptera exigua nuclear polyhedrosis virus. In Vitro Cell. Dev. BioI. 29A, 904907.
Hink, W.P. (1970) Established insect cell line from Trichoplusia ni. Nature 226,466-467.
Hink, W.P. and Ellis, B.J. (1971) Establishment and characterization of two new cell lines (CP-1268
and CP-169) from the codling moth, Carpocapsa pomonella. Curro Top. Microbiol. Immunol.
55,19-28.
Hink, W.P. and Ignoffo, C.M. (1970) Establishment of a new cell line (IMC-HZ-l) from ovaries of
cotton ballworm moth, Heliothis zea (boddie). Exp. Cell Res. 60, 307-309.
Inoue, H. and Mitsuhashi, J. (1984) A Bombyx mori cell line susceptible to a nuclear polyhedrosis
virus. J. Sericul. Sci. Jpn. 53,108-113.
Loeb, M.J. and Hakim, R.S. (1996) Insect midgut epithelium in vitro: an insect stem cell system. J.
Insect Physiol. 42, 11 03-1111.
Loeb, M.J., Jaffe, H., Gelman, D.B. and Hakim, R.S. (1999) Two polypeptide factors that promote
differentiation of insect midgut stem cells in vitro. Arch. Insect Biochem. Physiol. 40, 129-140.
Lynn, D.E., Miller, S.G. and Oberlander, H. (1982) Establishment of a cell line from lepidopteran
wing imaginal discs: induction of newly synthesized proteins by 20-hydroxyecdysone. Proc.
Nat! Acad. Sci. USA, BioI. Sci. 79,2589-2593.
Lynn, D.E. and Oberlander, H. (1983) The establishment of cell lines from imaginal wing discs of
Spodoptera frugiperda and Plodia interpunctella. J. Insect Physiol. 29, 591-596.
Lynn, D.E. and Oberlander, H. (1986) Obtainment of hormonally sensitive cell lines from imaginal
discs of Lepidoptera species. In: Kurstak, E (ed.) Techniques in the Life Sciences, Vol. C-2,
Techniques in in vitro invertebrate hormones and genes, C213, Elsevier Sci. Pub., County Clare,
1-12.
Mano, C. and Mitsuhashi, J. (1981)Primary culture of various tissues in the greater wax moth, Galleria mellonella (Insecta, Lepidoptera). Zool. Mag. 90, 76-84 (in Japanese with an English abstract).
Miltenburger, H.G., David, P., Mahr, U. and Zipp, W. (1977) Uber die Erstellung von LepidopterenDauerzellinien und die in vitro-Replikation von insektenpathogenen Vieren. I. Mamestra
brassicae 1. (Kohleule)- Zellinien und NPV-Replikation. Z. P. Ang. Entomol. 82,306-323.
Mitsuhashi, J. (1965) Tissue culture of the rice stem borer, Chilo suppressalis Walker (Lepidoptera:
Pyralidae). I. Cell migration from the explanted tissues of diapausing larvae. Jpn. J. App!.
Entomo!' Zool. 9, 137-141.
Mitsuhashi, J. (1972) Primary culture of hemocytopoietic tissue of Papilio xuthus Linne (Lepidoptera: Papilionidae). App!. Entomo!' Zoo!. 7, 39-41.