5 Insecta (Lepidoptera)
65
• • Procedure
1. Follow the instructions for sterilization as given in subprotocol 1.
Sterilization
1. Fix the insect dorsal side up, because the CNS runs along the ventral Dissection
midline.
2. Make an incision along the dorsal midline from the head to the last
abdominal segment.
3. Remove the CNS, from the brain to the last abdominal ganglion.
4. Remove the attached fat bodies, trachea and other tissues.
1. Wash the nervous system in physiological solution.
Culture
2. Transfer the nervous system into culture medium.
set-up
3. Cut the nerve cords so as to separate each ganglion.
4. Cut each ganglion into two to several pieces.
,
Elf CA
CC
. SG
.: ., • T1
• T2
i -T3
+, A1
,
A2
A3 "
•
~ A4
t A5
A6 ,.
A7 t
AB
Fig. 18. Central nervous system of the larva of the oriental tussock moth Euproctis subflava. Br, brain; CC, corpus cardiacum;
CA, corpus allatum; SG, suboesophageal ganglion; T i- T3, thoracic
ganglions; Ai-AS, abdominal ganglions. x 1.5
Fig. 19. Migrated cells from
a fragment of the brain (BR)
of the rice stem borer larva
Chilo suppressalis. x 150