5 Insecta (Lepidoptera)
63
• • Results
Fig. 15. Cell migration from
hemocytopoietic tissue (asterisk) of the swallowtail butterfly
Papilio xuthus. x ISO
Fig. 16. Monolayer of cells
formed by migrated cells from
hemocytopoietic tissue of the
swallowtail butterfly Papilio
xuthus. x ISO
Cell migration will occur rapidly. Usually, many cells come out from the
cut opening of the HPOs (Fig. 15). The migrated cells remain around the
explants at first, but will soon attach to the bottom of the vessel and may
form a monolayer (Fig. 16). The migrated cells can survive for more than
2 weeks, but multiplication barely occurs (Mitsuhashi, 1972). There are
no reports of the establishment of a continuous cell line from HPOs.
Subprotocol 10
Malpighian Tubes
Malpighian tubes open their ducts into the lumen of the gut at the pylorus, from which the tubes extend forward along the midgut (see chapter 5, Fig. 1). The Malpighian tubes are opaque zigzag-shaped thin tubes,
and are often somewhat colored; they are easily recognizable .
• • Materials
The equipment used is the same as that given for subprotocoll.
- Physiological solution
Equipment
Solutions