44
General Methods
Sending
frozen cells
1. Add a freeze protectant to the culture (see chapter 4, subprotocol 7
freezing step 2).
2. Freeze the cells according to the cryopreservation method.
3. Place the frozen tube in a box, and pack with dry ice.
4. Ship by air-mail or by another rapid transport system.
Results
During air transportation of cells, the temperature may not go down to
freezing point. However, if you send the cells to very cold area, the mail
may be exposed to very low temperatures that freeze the cells before they
are delivered to the recipient. If such a possibility is suspected, send frozen cells.
References
Miltenburger, H.G., Naser, W.L., and Schliermann, M.G. (1985) Establishment of a lepidopteran
hybrid cell line by use of a biochemical blocking method. In Vitro Cell. Dev. BioI. 21,433-438.
Rinaldini, L.M. (1959) An improved method for the isolation and quantitative cultivation of embryonic cells. Exp. Cell Res. 16,477-505.
Tapay, L.M., Lu, Y., Brock, J.A., Nadala, E.e.B. Jr and Loh, P.e. (1995) Transformation of primary
cultures of shrimp (Penaeus stylirostris) lymphoid (Oka) organ with Simian virus-40 (T) antigen. Proc. Soc. Exp. BioI. Med. 209, 73-78.
General Methods
Sending
frozen cells
1. Add a freeze protectant to the culture (see chapter 4, subprotocol 7
freezing step 2).
2. Freeze the cells according to the cryopreservation method.
3. Place the frozen tube in a box, and pack with dry ice.
4. Ship by air-mail or by another rapid transport system.
Results
During air transportation of cells, the temperature may not go down to
freezing point. However, if you send the cells to very cold area, the mail
may be exposed to very low temperatures that freeze the cells before they
are delivered to the recipient. If such a possibility is suspected, send frozen cells.
References
Miltenburger, H.G., Naser, W.L., and Schliermann, M.G. (1985) Establishment of a lepidopteran
hybrid cell line by use of a biochemical blocking method. In Vitro Cell. Dev. BioI. 21,433-438.
Rinaldini, L.M. (1959) An improved method for the isolation and quantitative cultivation of embryonic cells. Exp. Cell Res. 16,477-505.
Tapay, L.M., Lu, Y., Brock, J.A., Nadala, E.e.B. Jr and Loh, P.e. (1995) Transformation of primary
cultures of shrimp (Penaeus stylirostris) lymphoid (Oka) organ with Simian virus-40 (T) antigen. Proc. Soc. Exp. BioI. Med. 209, 73-78.