32
General Methods
• References
Fig. 1. A plastic filter holder (Sartorius)
Fig. 2. A filter system with a peristaltic pump. A, medium to be sterilized;
B, peristaltic pump; C, filter; D, sterilized medium reservoir
Fig. 3. Three small disposable filters attached
to syringes of appropriate sizes
Grace, T.D.C. (1962) Establishment of four strains of cells from insect tissues grown in vitro. Nature
195,788-789.
Maiorella, B., Inlow, D., Shauger, A. and Harano, D. (1988) Large-scale insect cell culture for recombinant protein production. Bio / Technology 6,1406-1410.
Mitsuhashi, J. (1989) Nutritional requirements of insect cells in vitro. In: Mitsuhashi, J. (ed) Invertebrate Cell System Applications, Vol. I. CRC, Boca Raton, pp 3-20.
Mitsuhashi, J. and Maramorosch, K. (1964) Leafhopper tissue culture: Embryonic, nymphal and
imaginal tissues from aseptic insects. Contrib. Boyce Thompson Inst. 22, 435-460.
Weiss, S.A., Smith, G.c., Kalter, S.S. and Vaughn, J.L. (1981) Improved method for the production of
insect cell cultures in large volume. In Vitro 17,495-502.