Composition of Salt Solutions and Culture Media
419
2,000, MgS0 4 ·7H 2 0 1,230, NaCI3,200, NaH 2 P0 4 ·H 2 0 420, CuS0 4 0.001, FeS0 4 ·7H 2 0 1.0,
MnS0 4 ·7H 2 0 1.0, ZnS0 4 ·7H 2 0 1.0, biotin 0.05, calcium pantothenate 5.0, choline chloride 50.0, folic acid 0.05, m-inositoI50.0, niacinamide 0.5, pyridoxal hydrochloride 5.0,
riboflavin 0.01, thiamine hydrochloride 5.0, cyanocobalamin 0.05, cholesterol 0.01, linoleic acid 0.01, and phenol red 10.0, made up to 1,000 ml with distilled water. The pH
of the medium is adjusted to 6.6 with 1 N NaOH. The final osmolarity of the medium is
250 mOsmollkg.
References
Ballard, T.A., Roer, R.D. and Dillaman, R.M. (1993) J. Tissue Cult. Methods 15, 11-14.
Basch P.F. and DiConza, J.J. (1973) Am. J. Trop. Med. Hygiene 22, 805-813.
Bayer, D.S. and Barlow, R.B. Jr. (1978) J. Gen. Physiol. 72, 539-563.
Betchaku, T. (1967) J. Exp. Zool. 164,407-434.
Burnett, A.L., Ruffing, F.E., Zongker, J. and Necco, A. (1968) J. Embryol. Exp. Morphol. 20, 73-80.
Carlson, J.G., Hollander, A., and Gaulden, M.E. (1947) Science 105,187-188.
Chernin, E. (1963) J. Parasitol, 49, 353-364.
Chiarandini, D.J. (1964) Life Sci. 3,1513-1518.
Cooke, I., Graf, R., Grau, S., Haylett, B., Meyers, D. and Ruben, P. (1989) Proc. Natl Acad. Sci. USA 86,
402-406.
Daig, K. and Spindler, K.-D. (1979) Z. Naturforsch. 34C, 1243-1247.
Davis, K.T. and Shearn, A. (1977) Science 196,438-440.
Dieckmann, A. and Frank, W. (1988) Parasitol. Res. 74, 297-298.
Domart-Coulon, I., Doumenc, D., Auzoux-Bordenave, S. and Le Fichant, Y. (1994) Cytotechnology
16,109-120.
Durchon, M. and Schaller, F. (1963) e. R. Acad. Sci. 256, 5615-5617.
Furuta, E. and Shimozawa, A. (1983) Zool. Mag. 92, 290-296.
Fyhn, U.E.H. and Costlow, J.D. (1975) BioI. Bull. 149,316-330.
Gey, G.O. (1949) Bull. Tissue Culture Assoc. March 16.
Gottschewski, G.H.M. (1960) Roux's Arch. Entwicklungsmech. 152,204-229.
Grace, T.D.e. (1962) Nature 195,788-789.
Hansen, E.L. (1979) Tissue Culture Assoc. ManualS, 1009-1014.
Heacox A.E., Fisher, A. and Frangenberg, H.-R. (1983) In Vitro 19,825-832.
Horikawa, M. (1959) Cytologia 23, 468-477.
Holtfreter, J. (1931) Roux's Arch. Entwicklungsmech. Organ. 124,404-467.
Ivic, 1.., Nesic, 0., Kartelija, G. and Pasic, M. (1995) Compo Biochem. Physiol. 111C, 413-421.
Iwanaga, Y., Tsuji, M. and Oda, S. (1985) Venus 44,103-109. (in Japanese with English abstract)
Kaatz, H.H., Hagedorn, H.H. and Engels, W. (1985) In Vitro Cell. Dev. BioI. 21, 347-352.
Kuroda, Y. and Tamura, S. (1955) Zool. Mag. Jpn. 64, 380-384. (in Japanese with English abstract)
Landureau, J.e. and Grellet, P. (1972) e. R. Acad. Sci. D274, 1372-1375.
Laverdure, A.-M. (1971) Gen. Compo Endocrinol. 17,467-478.
Lebel, J.-M., Giard, W, Favrel, P. and Boucaud-Camou, E. (1996) BioI. Cell. 86, 67-72.
Leibovitz, A. (1963) Am. J. Hygiene 78, 173-183.
Leloup, A.-M. (1964) Bull. Soc. Zool. Fr. 89, 70-77.
Locke, F.S. (1895) J. Boston Sci. Med. 1,2-3.
Loomis, WF. and Lenhoff, H.M. (1956) J. Exp. Zool. 132,555-568.
Mcintosh, A.H., Maramorosch, K. and Rectoris, e. (1973) In Vitro 8,375-378.
Machii, A. and Wada, K.T. (1989) In: Mitsuhashi J. (ed) Invertebrate Cell System Applications, Vol. II.
CRC, Boca Raton, pp 225-233.
Miltenburger, H.G., Naser, W.L., Harvey, J.P., Huber, J. and Huger, A.M. (1984) Z. Naturforsch. 39C,
993-1002.
Mitsuhashi, J. (1967) Nature 215, 863-864.
Mitsuhashi, J. (1980) In: Kurstak, E., Maramorosch, K. and Dtibendorfer, A. (eds) Invertebrate Systems In Vitro. Elsevier/North Holland Biomedical, Amsterdam, pp 47-58.
419
2,000, MgS0 4 ·7H 2 0 1,230, NaCI3,200, NaH 2 P0 4 ·H 2 0 420, CuS0 4 0.001, FeS0 4 ·7H 2 0 1.0,
MnS0 4 ·7H 2 0 1.0, ZnS0 4 ·7H 2 0 1.0, biotin 0.05, calcium pantothenate 5.0, choline chloride 50.0, folic acid 0.05, m-inositoI50.0, niacinamide 0.5, pyridoxal hydrochloride 5.0,
riboflavin 0.01, thiamine hydrochloride 5.0, cyanocobalamin 0.05, cholesterol 0.01, linoleic acid 0.01, and phenol red 10.0, made up to 1,000 ml with distilled water. The pH
of the medium is adjusted to 6.6 with 1 N NaOH. The final osmolarity of the medium is
250 mOsmollkg.
References
Ballard, T.A., Roer, R.D. and Dillaman, R.M. (1993) J. Tissue Cult. Methods 15, 11-14.
Basch P.F. and DiConza, J.J. (1973) Am. J. Trop. Med. Hygiene 22, 805-813.
Bayer, D.S. and Barlow, R.B. Jr. (1978) J. Gen. Physiol. 72, 539-563.
Betchaku, T. (1967) J. Exp. Zool. 164,407-434.
Burnett, A.L., Ruffing, F.E., Zongker, J. and Necco, A. (1968) J. Embryol. Exp. Morphol. 20, 73-80.
Carlson, J.G., Hollander, A., and Gaulden, M.E. (1947) Science 105,187-188.
Chernin, E. (1963) J. Parasitol, 49, 353-364.
Chiarandini, D.J. (1964) Life Sci. 3,1513-1518.
Cooke, I., Graf, R., Grau, S., Haylett, B., Meyers, D. and Ruben, P. (1989) Proc. Natl Acad. Sci. USA 86,
402-406.
Daig, K. and Spindler, K.-D. (1979) Z. Naturforsch. 34C, 1243-1247.
Davis, K.T. and Shearn, A. (1977) Science 196,438-440.
Dieckmann, A. and Frank, W. (1988) Parasitol. Res. 74, 297-298.
Domart-Coulon, I., Doumenc, D., Auzoux-Bordenave, S. and Le Fichant, Y. (1994) Cytotechnology
16,109-120.
Durchon, M. and Schaller, F. (1963) e. R. Acad. Sci. 256, 5615-5617.
Furuta, E. and Shimozawa, A. (1983) Zool. Mag. 92, 290-296.
Fyhn, U.E.H. and Costlow, J.D. (1975) BioI. Bull. 149,316-330.
Gey, G.O. (1949) Bull. Tissue Culture Assoc. March 16.
Gottschewski, G.H.M. (1960) Roux's Arch. Entwicklungsmech. 152,204-229.
Grace, T.D.e. (1962) Nature 195,788-789.
Hansen, E.L. (1979) Tissue Culture Assoc. ManualS, 1009-1014.
Heacox A.E., Fisher, A. and Frangenberg, H.-R. (1983) In Vitro 19,825-832.
Horikawa, M. (1959) Cytologia 23, 468-477.
Holtfreter, J. (1931) Roux's Arch. Entwicklungsmech. Organ. 124,404-467.
Ivic, 1.., Nesic, 0., Kartelija, G. and Pasic, M. (1995) Compo Biochem. Physiol. 111C, 413-421.
Iwanaga, Y., Tsuji, M. and Oda, S. (1985) Venus 44,103-109. (in Japanese with English abstract)
Kaatz, H.H., Hagedorn, H.H. and Engels, W. (1985) In Vitro Cell. Dev. BioI. 21, 347-352.
Kuroda, Y. and Tamura, S. (1955) Zool. Mag. Jpn. 64, 380-384. (in Japanese with English abstract)
Landureau, J.e. and Grellet, P. (1972) e. R. Acad. Sci. D274, 1372-1375.
Laverdure, A.-M. (1971) Gen. Compo Endocrinol. 17,467-478.
Lebel, J.-M., Giard, W, Favrel, P. and Boucaud-Camou, E. (1996) BioI. Cell. 86, 67-72.
Leibovitz, A. (1963) Am. J. Hygiene 78, 173-183.
Leloup, A.-M. (1964) Bull. Soc. Zool. Fr. 89, 70-77.
Locke, F.S. (1895) J. Boston Sci. Med. 1,2-3.
Loomis, WF. and Lenhoff, H.M. (1956) J. Exp. Zool. 132,555-568.
Mcintosh, A.H., Maramorosch, K. and Rectoris, e. (1973) In Vitro 8,375-378.
Machii, A. and Wada, K.T. (1989) In: Mitsuhashi J. (ed) Invertebrate Cell System Applications, Vol. II.
CRC, Boca Raton, pp 225-233.
Miltenburger, H.G., Naser, W.L., Harvey, J.P., Huber, J. and Huger, A.M. (1984) Z. Naturforsch. 39C,
993-1002.
Mitsuhashi, J. (1967) Nature 215, 863-864.
Mitsuhashi, J. (1980) In: Kurstak, E., Maramorosch, K. and Dtibendorfer, A. (eds) Invertebrate Systems In Vitro. Elsevier/North Holland Biomedical, Amsterdam, pp 47-58.