Composition of Salt Solutions and Culture Media
415
Pf-35 medium (Machii and Wada, 1989)
Unless otherwise indicated, the concentrations are given as mg/l,OOO ml culture medium.
NaCI26,220.0, KCll,080.0, MgS04 3,130.0, MgCl 2 2,200.0, CaCl 2 1,120.0, NaHC0 3 300.0,
NaH 2 P0 4 44.0, L-cystine 0.7, L-tyrosine 3.6, taurine 128.9, L-aspartic acid 12.0, L-threonine 3.6, L-serine 9.5, L-asparagine 5.3, L-glutamic acid 19.1, L-glutamine 17.5, L-proline
3.5, glycine 15.0, L-a-alanine 17.8, a-amino-n-butyric acid 0.8, L-valine 4.7, L-methionine 3.0, L-isoleucine 2.6, L-Ieucine 3.9, L-phenylalanine 1.7, L-ornithine 0.9, L-Iysine 7.3,
L-tryptophan 1.0, L-histidine 4.7, L-arginine 17.4, phosphoserine 1.0,p-aminobenzoic
acid 0.05, biotin 0.01, calcium pantothenate 1.0, choline chloride 250.0, folic acid 0.01,
inositol 0.05, niacin 0.03, niacinamide 5.0, pyridoxal hydrochloride 0.025, pyridoxine
hydrochloride 1.0, riboflavin 1.0, cyanocobalamin 0.01, thiamine hydrochloride 10.0,
ascorbic acid 40.0, vitamin A 0.1, a-tocopherol acetate 0.01, calciferol 0.1, menadione
0.01, adenine 10.0, guanine hydrochloride 0.3, hypoxanthine 0.3, thymine 0.3, uracil
0.3, xanthine 0.3, adenylic acid 0.2, 2-deoxy-o-ribose 0.5, o-ribose 0.5, Tween-80 5.0,
cholesterol 0.2, glutathione 0.05, sodium acetate 50.0, glucose 300, ATP 10.0, lactalbumin hydrolysate 100.0, phenol red 5.0, penicillin 200, kanamycin 100, and FBS 100 ml,
made up to 1,000 ml with distilled water.
Planarian cell culture medium (Betchaku, 1967)
Unless otherwise indicated, the concentrations are given as mgll,OOO ml culture medium.
L-Arginine hydrochloride 5.6, L-cystine 2.0, L-histidine 1.1, L-isoleucine 8.7, L-Ieucine
4.7, L-Iysine 6.1, L-methionine 2.7, L-phenylalanine 2.8, L-threonine 4.0, L-tryptophan
0.7, L-tyrosine 6.0, L-valine 3.9, L-glutamine 48.9, choline chloride 0.3, nicotinamide
0.3, calcium-o-pantothenate 0.3, pyridoxal hydrochloride 0.3, riboflavin 0.03, thiamine
hydrochloride 0.3, i-inositol 0.3, biotin 0.3, folic acid 0.3, sodium pyruvate 150.0, glucose 300.0, calf serum 3 ml, NaCll,750.0, KCI25.0, CaCl 2 50.0, NaHC0 3 100.0, neomycin 100.0. The pH of the medium should be adjusted to 7.2-7.4 with 1 N KOH and 1 N
HCl.
Rasmont medium M (Rasmont, 1961)
The concentrations are given as milliequivalent II.
Ca++ 2.00, Mg++ 1.00, Na+ 1.00, K+ 0.05, Cl~ 2.05, S04 1.00, Si03~~ 0.50, HC03~ 0.50.
RML-375 medium (Moulton, 1978)
AI: 1 : 2 mixture of Eagle's MEM, Hank's BSS and Leibovitz's L-15 medium is supplemented with 20% FBS (heat-inactivated for 1 h at 56°C), 0.1% bovine albumin, 10%
tryptose phosphate broth stock solution, made by dissolving 109 tryptose phosphate
broth in 100 ml distilled water, and 50 glml gentamicin.
S-20 medium (Landureau and Grellet, 1972)
The concentrations are given as mg/1,OOO ml culture medium.
NaCl 8,474, KCI 7,070, MgS04·7H20 1,016, CaCl2 222, MnS04·H 2 0 32, P03H3 900, glucose 4,000, L-arginine hydrochloride 800, L-aspartic acid 506, L-cysteine hydrochloride
646, L-glutamic acid 1,500, glycine 750, L-histidine 202, L-isoleucine 131, L-Ieucine 197,
L-Iysine hydrochloride 183, L-methionine 254, L-phenylalanine 99, L-proline 750, L-serine
32, L-threonine 100, L-tryptophan 102, L-tyrosine 90, L-valine 94, folic acid 0.02, d-biotin 0.01, choline chloride 181.48, cyanocobalamine 0.04, inositol 0.05, nicotinamide
0.04, pyridoxine hydrochloride 0.04, riboflavin 0.19, thiamine hydrochloride 0.67, cal-
415
Pf-35 medium (Machii and Wada, 1989)
Unless otherwise indicated, the concentrations are given as mg/l,OOO ml culture medium.
NaCI26,220.0, KCll,080.0, MgS04 3,130.0, MgCl 2 2,200.0, CaCl 2 1,120.0, NaHC0 3 300.0,
NaH 2 P0 4 44.0, L-cystine 0.7, L-tyrosine 3.6, taurine 128.9, L-aspartic acid 12.0, L-threonine 3.6, L-serine 9.5, L-asparagine 5.3, L-glutamic acid 19.1, L-glutamine 17.5, L-proline
3.5, glycine 15.0, L-a-alanine 17.8, a-amino-n-butyric acid 0.8, L-valine 4.7, L-methionine 3.0, L-isoleucine 2.6, L-Ieucine 3.9, L-phenylalanine 1.7, L-ornithine 0.9, L-Iysine 7.3,
L-tryptophan 1.0, L-histidine 4.7, L-arginine 17.4, phosphoserine 1.0,p-aminobenzoic
acid 0.05, biotin 0.01, calcium pantothenate 1.0, choline chloride 250.0, folic acid 0.01,
inositol 0.05, niacin 0.03, niacinamide 5.0, pyridoxal hydrochloride 0.025, pyridoxine
hydrochloride 1.0, riboflavin 1.0, cyanocobalamin 0.01, thiamine hydrochloride 10.0,
ascorbic acid 40.0, vitamin A 0.1, a-tocopherol acetate 0.01, calciferol 0.1, menadione
0.01, adenine 10.0, guanine hydrochloride 0.3, hypoxanthine 0.3, thymine 0.3, uracil
0.3, xanthine 0.3, adenylic acid 0.2, 2-deoxy-o-ribose 0.5, o-ribose 0.5, Tween-80 5.0,
cholesterol 0.2, glutathione 0.05, sodium acetate 50.0, glucose 300, ATP 10.0, lactalbumin hydrolysate 100.0, phenol red 5.0, penicillin 200, kanamycin 100, and FBS 100 ml,
made up to 1,000 ml with distilled water.
Planarian cell culture medium (Betchaku, 1967)
Unless otherwise indicated, the concentrations are given as mgll,OOO ml culture medium.
L-Arginine hydrochloride 5.6, L-cystine 2.0, L-histidine 1.1, L-isoleucine 8.7, L-Ieucine
4.7, L-Iysine 6.1, L-methionine 2.7, L-phenylalanine 2.8, L-threonine 4.0, L-tryptophan
0.7, L-tyrosine 6.0, L-valine 3.9, L-glutamine 48.9, choline chloride 0.3, nicotinamide
0.3, calcium-o-pantothenate 0.3, pyridoxal hydrochloride 0.3, riboflavin 0.03, thiamine
hydrochloride 0.3, i-inositol 0.3, biotin 0.3, folic acid 0.3, sodium pyruvate 150.0, glucose 300.0, calf serum 3 ml, NaCll,750.0, KCI25.0, CaCl 2 50.0, NaHC0 3 100.0, neomycin 100.0. The pH of the medium should be adjusted to 7.2-7.4 with 1 N KOH and 1 N
HCl.
Rasmont medium M (Rasmont, 1961)
The concentrations are given as milliequivalent II.
Ca++ 2.00, Mg++ 1.00, Na+ 1.00, K+ 0.05, Cl~ 2.05, S04 1.00, Si03~~ 0.50, HC03~ 0.50.
RML-375 medium (Moulton, 1978)
AI: 1 : 2 mixture of Eagle's MEM, Hank's BSS and Leibovitz's L-15 medium is supplemented with 20% FBS (heat-inactivated for 1 h at 56°C), 0.1% bovine albumin, 10%
tryptose phosphate broth stock solution, made by dissolving 109 tryptose phosphate
broth in 100 ml distilled water, and 50 glml gentamicin.
S-20 medium (Landureau and Grellet, 1972)
The concentrations are given as mg/1,OOO ml culture medium.
NaCl 8,474, KCI 7,070, MgS04·7H20 1,016, CaCl2 222, MnS04·H 2 0 32, P03H3 900, glucose 4,000, L-arginine hydrochloride 800, L-aspartic acid 506, L-cysteine hydrochloride
646, L-glutamic acid 1,500, glycine 750, L-histidine 202, L-isoleucine 131, L-Ieucine 197,
L-Iysine hydrochloride 183, L-methionine 254, L-phenylalanine 99, L-proline 750, L-serine
32, L-threonine 100, L-tryptophan 102, L-tyrosine 90, L-valine 94, folic acid 0.02, d-biotin 0.01, choline chloride 181.48, cyanocobalamine 0.04, inositol 0.05, nicotinamide
0.04, pyridoxine hydrochloride 0.04, riboflavin 0.19, thiamine hydrochloride 0.67, cal-