410
Appendix
K-6 medium (Horikawa, 1959)
The concentrations are given as mg/l,OOO ml culture medium.
NaCl 7,000, KC1200, CaCI 2 ·2H 2 0 20, MgCI 2 ·6H 2 0 100, NaHC0 3 50, NaH 2 P0 4 ·2H 2 0 200,
glucose 800, casein hydrolysate 50,000, tryptophan 5,000, cystine 1,000, cysteine 260,
oL-kynurenine 4 g, oL·3-hydroxykynurenine 2 g, ribonucleic acid 500, thiamine 0.05,
riboflavin 0.05, pyridoxine hydrochloride 0.125, niacinamide 0.125, calcium pantothenate 0.05, ascorbic acid 250, biotin 0.05, folic acid 0.05, choline chloride 2.5, inosite
0.25,p-aminobenzoic acid 0.25, vitamin A 0.5, vitamin B12 0.5, sodium acetate SO, glutathione 10, glutamic acid 100, diphosphopyridine nucleotide (DPN) 1.0. The pH of
the medium should be adjusted to 7.2 with 1 N KOH.
Kuroda and Tamura's medium for Drosophila melanotic tumors (Kuroda and Tamura,
1955)
The concentrations are given as mg/1,OOO ml culture medium.
NaCI 7,000, KC1200, CaCI 2 ·2H 2 0 20, MgCI2·6H20100, NaHC0 3 SO, NaH 2 P0 4 ·2Hp 200,
glucose 800, casein hydrolysate 50,000, tryptophan 1,000, cystine 1,000, cysteine 260,
ribonucleic acid 500, thiamine 0.05, riboflavin 0,05, pyridoxine hydrochloride 0.125,
niacinamide 0.125, calcium pantothenate 0.05, ascorbic acid 250, biotin 0.05, folic acid
0.05, choline chloride 2.5, inosite 0.25, p-aminobenzoic acid 0.25, cyanocobalamin 0.5,
vitamin A 0.5, sodium acetate SO, glutathione 10, glutamic acid 100, and DPN 1.0.
L-15 medium (Leibovitz, 1963)
The concentrations are given as mg/1 ,000 ml culture medium.
NaCI 8,000, KCI 400, MgS04·7H20 200, MgCl2·6H20 200, CaCl2 140, Na2HP04 190,
KH 2 P0 4 60, oL-a-alanine 450, L-arginine 500, L-asparagine 250, L-cysteine 120, glycine
200, L-histidine 250, DL-isoleucine 250, L-Ieucine 125, L-Iysine 75, DL-methionine 150,
DL-phenylalanine 250, L-serine 200, DL-threonine 600, L-tryptophan 20, DL-valine 200,
choline 1, folic acid 1, inositol 2, nicotinamide 1, DL-Ca pantothenate 1, pyridoxine
hydrochloride 1, sodium riboflavin-5-phosphate 0.1, thiamine monophosphate 1, Lglutamine 300, L-tyrosine 300, sodium pyruvate 550, o{ + )galactose 900, and phenol
red 10.
L-15B (Munderloh and Kurtti, 1989)
Unless otherwise indicated, the concentrations are given as mg/1 ,000 ml culture medium.
Leibovitz's L-15 medium powder (Gibco) 1 package 03.88g), L-aspartic acid 299, Lglutamic acid 500, L-glutamine 292, L-proline 300, a-ketoglutaric acid 299, o-glucose
2,239, mineral stock* 1 ml, vitamin stock** 1 ml. The pH of the medium is adjusted to
6.4-6.6 with 1 N NaOH.
*Mineral stock (x10 3 ) contains stock solutions A, B, and C. The components of stock
solution A (xl0S) are: CoCI·6H 2 0 20 mg, CuS0 4 ·5H 2 0 20 mg, MnS0 4 ·H 2 0 160 mg,
ZnS0 4 ·7H 2 0 200 mg, and distilled water 100 ml. The components of stock solution B
(xlO S ) are NaMo0 4 ·2H 2 0 20 mg and distilled water 100 ml. The components of stock
solution C (xlO S ) are Na 2 Se0 3 20 mg and distilled water 100 ml. The complete mineral
stock (xl03) are: reduced glutathione 1,000 mg, ascorbic acid 1,000 mg, FeS0 4 ·7H 2 0
50 mg, stock solution A 1 ml, stock solution B 1 ml, stock solution C 1 ml, and distilled
water to 100 ml.
**Vitamin stock (xl0 3 ): p-aminobenzoic acid 100 mg, cyanocobalamin 50 mg, o-biotin 10 mg, and distilled water 100 ml.
Appendix
K-6 medium (Horikawa, 1959)
The concentrations are given as mg/l,OOO ml culture medium.
NaCl 7,000, KC1200, CaCI 2 ·2H 2 0 20, MgCI 2 ·6H 2 0 100, NaHC0 3 50, NaH 2 P0 4 ·2H 2 0 200,
glucose 800, casein hydrolysate 50,000, tryptophan 5,000, cystine 1,000, cysteine 260,
oL-kynurenine 4 g, oL·3-hydroxykynurenine 2 g, ribonucleic acid 500, thiamine 0.05,
riboflavin 0.05, pyridoxine hydrochloride 0.125, niacinamide 0.125, calcium pantothenate 0.05, ascorbic acid 250, biotin 0.05, folic acid 0.05, choline chloride 2.5, inosite
0.25,p-aminobenzoic acid 0.25, vitamin A 0.5, vitamin B12 0.5, sodium acetate SO, glutathione 10, glutamic acid 100, diphosphopyridine nucleotide (DPN) 1.0. The pH of
the medium should be adjusted to 7.2 with 1 N KOH.
Kuroda and Tamura's medium for Drosophila melanotic tumors (Kuroda and Tamura,
1955)
The concentrations are given as mg/1,OOO ml culture medium.
NaCI 7,000, KC1200, CaCI 2 ·2H 2 0 20, MgCI2·6H20100, NaHC0 3 SO, NaH 2 P0 4 ·2Hp 200,
glucose 800, casein hydrolysate 50,000, tryptophan 1,000, cystine 1,000, cysteine 260,
ribonucleic acid 500, thiamine 0.05, riboflavin 0,05, pyridoxine hydrochloride 0.125,
niacinamide 0.125, calcium pantothenate 0.05, ascorbic acid 250, biotin 0.05, folic acid
0.05, choline chloride 2.5, inosite 0.25, p-aminobenzoic acid 0.25, cyanocobalamin 0.5,
vitamin A 0.5, sodium acetate SO, glutathione 10, glutamic acid 100, and DPN 1.0.
L-15 medium (Leibovitz, 1963)
The concentrations are given as mg/1 ,000 ml culture medium.
NaCI 8,000, KCI 400, MgS04·7H20 200, MgCl2·6H20 200, CaCl2 140, Na2HP04 190,
KH 2 P0 4 60, oL-a-alanine 450, L-arginine 500, L-asparagine 250, L-cysteine 120, glycine
200, L-histidine 250, DL-isoleucine 250, L-Ieucine 125, L-Iysine 75, DL-methionine 150,
DL-phenylalanine 250, L-serine 200, DL-threonine 600, L-tryptophan 20, DL-valine 200,
choline 1, folic acid 1, inositol 2, nicotinamide 1, DL-Ca pantothenate 1, pyridoxine
hydrochloride 1, sodium riboflavin-5-phosphate 0.1, thiamine monophosphate 1, Lglutamine 300, L-tyrosine 300, sodium pyruvate 550, o{ + )galactose 900, and phenol
red 10.
L-15B (Munderloh and Kurtti, 1989)
Unless otherwise indicated, the concentrations are given as mg/1 ,000 ml culture medium.
Leibovitz's L-15 medium powder (Gibco) 1 package 03.88g), L-aspartic acid 299, Lglutamic acid 500, L-glutamine 292, L-proline 300, a-ketoglutaric acid 299, o-glucose
2,239, mineral stock* 1 ml, vitamin stock** 1 ml. The pH of the medium is adjusted to
6.4-6.6 with 1 N NaOH.
*Mineral stock (x10 3 ) contains stock solutions A, B, and C. The components of stock
solution A (xl0S) are: CoCI·6H 2 0 20 mg, CuS0 4 ·5H 2 0 20 mg, MnS0 4 ·H 2 0 160 mg,
ZnS0 4 ·7H 2 0 200 mg, and distilled water 100 ml. The components of stock solution B
(xlO S ) are NaMo0 4 ·2H 2 0 20 mg and distilled water 100 ml. The components of stock
solution C (xlO S ) are Na 2 Se0 3 20 mg and distilled water 100 ml. The complete mineral
stock (xl03) are: reduced glutathione 1,000 mg, ascorbic acid 1,000 mg, FeS0 4 ·7H 2 0
50 mg, stock solution A 1 ml, stock solution B 1 ml, stock solution C 1 ml, and distilled
water to 100 ml.
**Vitamin stock (xl0 3 ): p-aminobenzoic acid 100 mg, cyanocobalamin 50 mg, o-biotin 10 mg, and distilled water 100 ml.