292
Organ Culture
several layers, which are, from the bottom of the centrifuge tube, sex
cells and a layer of erythrocytes, a layer of viscous agglutinated leukocytes, a thin white layer of enigmatic vesicles and a fraction of the
urns, and finally a layer of colorless plasma containing some urns.
Culture 1. Take the layer of enigmatic vesicles and urns, and suspend this layer
set-up
in the plasma, and place this mixture in culture vessels.
Results
According to Thomas (l932a,b), during the early phase of culture, the
urns and vesicles multiply. The organs could be subcultured after a period ranging from 3 weeks to 1 month. The urns continued to exhibit
activity and move about as a result of beating cilia for 3 months. The
enigmatic vesicles persisted for 6 months.
• References
Berjon, J.-J. (1965) Application de la culture organotypique sur milieux artificiels a la discrimination des fonctions endocrines des ganglions cerebroi"des du Lombricien Eisenia foetida (Sav.).
C. R. Acad. Sci. 260, 6212-6214.
Durchon, M. (1963) Resultats de cultures organotypiques chez les annelides et les mollusques
gasteropodes. Bull. Soc. Zool. Fr. 89, 45-48.
Durchon, M. and Boilly, B. (1964) Etude ultrastructurale de l'influence de l'hormone cerebrale des
Nereidiens sur Ie developpement des ovocytes de Nereis diversicolor O.F. Muller (Annelide
Polychete) en culture organotypique. C. R. Acad. Sci. 259,1245-1247.
Durchon, M. and Dhainaut, A. (1964) Influence de l'hormone cerebrale des Nereidiens sur la
croissance des ovocytes. Etude en culture organotypique. C. R. Acad. Sci. 259, 917-919.
Durchon, M. and Schaller, F. (1963) Application de la methode de culture organotypique aux
recherches endocrinologiques chez les Annelides Polychetes. C. R. Acad Sci. 256, 5615-5617.
Malecha, J. (1965) Culture organotypique d'Hirudinees. C. R. Soc. BioI. 159, 1674-1675.
Malecha, J. (1967a) Etude en culture organotypique de l'influence endocrine de la masse nerveuse
peripharyngienne sur la maturation testiculaire chez Hirudo medicinalis L. C. R. Acad. Sci.
265,1806-1808.
Malecha, J. (1967b) Transformation heteronereidienne et gametogenese chez Nereis succinea
(Leuckart) (Annelide Polychete) en culture organotypique. C. R. Acad. Sci. 265, 613-615.
Marcel, R. (1966) Role inducteur du cerveau au cours de la regeneration du pharynx chez Eisenia
foetida Say. (Annelide Oligochete). C. R. Acad. Sci. 262, 2470-2472.
Thomas, J.-A. (1932a) La culture des vesicles enigmatiques et des urnes du Siponcle. C. R. Soc. BioI.
110,451-452.
Thomas, I.-A. (1932b) Recherches cytologiques et experimentales sur les vesicules enigmatiques et
les urnes du siponcle. Arch. Zool. Exp. Gen. 73, 22-40.
Organ Culture
several layers, which are, from the bottom of the centrifuge tube, sex
cells and a layer of erythrocytes, a layer of viscous agglutinated leukocytes, a thin white layer of enigmatic vesicles and a fraction of the
urns, and finally a layer of colorless plasma containing some urns.
Culture 1. Take the layer of enigmatic vesicles and urns, and suspend this layer
set-up
in the plasma, and place this mixture in culture vessels.
Results
According to Thomas (l932a,b), during the early phase of culture, the
urns and vesicles multiply. The organs could be subcultured after a period ranging from 3 weeks to 1 month. The urns continued to exhibit
activity and move about as a result of beating cilia for 3 months. The
enigmatic vesicles persisted for 6 months.
• References
Berjon, J.-J. (1965) Application de la culture organotypique sur milieux artificiels a la discrimination des fonctions endocrines des ganglions cerebroi"des du Lombricien Eisenia foetida (Sav.).
C. R. Acad. Sci. 260, 6212-6214.
Durchon, M. (1963) Resultats de cultures organotypiques chez les annelides et les mollusques
gasteropodes. Bull. Soc. Zool. Fr. 89, 45-48.
Durchon, M. and Boilly, B. (1964) Etude ultrastructurale de l'influence de l'hormone cerebrale des
Nereidiens sur Ie developpement des ovocytes de Nereis diversicolor O.F. Muller (Annelide
Polychete) en culture organotypique. C. R. Acad. Sci. 259,1245-1247.
Durchon, M. and Dhainaut, A. (1964) Influence de l'hormone cerebrale des Nereidiens sur la
croissance des ovocytes. Etude en culture organotypique. C. R. Acad. Sci. 259, 917-919.
Durchon, M. and Schaller, F. (1963) Application de la methode de culture organotypique aux
recherches endocrinologiques chez les Annelides Polychetes. C. R. Acad Sci. 256, 5615-5617.
Malecha, J. (1965) Culture organotypique d'Hirudinees. C. R. Soc. BioI. 159, 1674-1675.
Malecha, J. (1967a) Etude en culture organotypique de l'influence endocrine de la masse nerveuse
peripharyngienne sur la maturation testiculaire chez Hirudo medicinalis L. C. R. Acad. Sci.
265,1806-1808.
Malecha, J. (1967b) Transformation heteronereidienne et gametogenese chez Nereis succinea
(Leuckart) (Annelide Polychete) en culture organotypique. C. R. Acad. Sci. 265, 613-615.
Marcel, R. (1966) Role inducteur du cerveau au cours de la regeneration du pharynx chez Eisenia
foetida Say. (Annelide Oligochete). C. R. Acad. Sci. 262, 2470-2472.
Thomas, J.-A. (1932a) La culture des vesicles enigmatiques et des urnes du Siponcle. C. R. Soc. BioI.
110,451-452.
Thomas, I.-A. (1932b) Recherches cytologiques et experimentales sur les vesicules enigmatiques et
les urnes du siponcle. Arch. Zool. Exp. Gen. 73, 22-40.