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face of the culture medium, bringing the tracheal system in contact
with the air.
2. Wash the explants several times with medium containing 2.5 mg/
100 ml phenylthiourea to prevent melanization.
3. Keep the culture at 34SC.
Results
According to Kaatz et al. (1985), protein synthesis was maintained in cultured fat bodies or ovaries. The rate of synthesis increased markedly when
the tracheal system of the organs was left intact and open to the air during incubation.
Subprotocol 18
Regenerating Tissues from Cockroach Legs (Blatta ria)
Materials
- Dissecting microscope and dissecting tray (see chapter 1, section 3 Equipment
"Tools")
- Scissors
- Forceps
- Needles
- Maximov slide
- Pasteur pipettes
- Rose multipurpose culture system (Ikemoto Rika)
- Dialysis strips
- Physiological solution
- Leucophaea culture medium (see Appendix 1)
- Regenerating tissues of cockroach legs (see Comments)
Procedure
Solutions
Animals
1. Aseptically dissect out the regenerating tissues.
Dissection
2. Trim and rinse the dissected tissues with physiological solution.
1. Transfer the tissues to Rose multipurpose culture chambers under di- Culture
alysis strips.
set-up
2. Circulate the culture medium.
Comments
Regenerating tissues: in order to prepare regenerating tissue, Marks and
Reinecke (1964) adopted following procedure.