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octomerous anthozoan
CtQU MC-tiOn A
9
hexamerous anthozoan
relreClor
musde
sulcus
primary
saptum
secondary
""Plum
exocoet
endoooel
Fig. 2. Schema of the inner structure of a sea anemone. From Stachowitsch, M.
"The Invertebrates", © 1992. Reproduced by permission of John Wiley & Sons, Inc.
- Culture medium (95 ml seawater, 5 ml distilled water, 0.02% NaHC0 3 , Solutions
0.25% glucose, and 0.1 % peptone)
- Sea anemone
Animals
• • Procedure
1. Surface sterilize the animal.
2. Remove the septa (Fig. 2), and cut it into small pieces.
1. Transfer the tissue fragments to culture dishes.
2. Add culture medium .
• • Results
Lewis (1916) cultured pieces of the septa. The explants gave rise to an
abundant growth, which was composed of substances that were probably mesogloea and numerous scattered endoderm cells. The cilia along
the edge of the septa remained active for many days in the culture.
Subprotocol 4
Cells from Colonial Cnidarians (Anthozoa and Hydrozoa)
• • Materials
- Aerated aquarium
- Humidified incubator
Dissection
Culture
set-up
Equipment