14 Echinodermata
157
periproct /terminal tentacle
anus I / /oCUlar plate
I
/.
aboral perihemal
-..... ..
ring
..
aboral hemal ring
axial gland
stonecanal===t==~~~~~~~~~~~~~~~~
polian
vesicle
radial nerve
radial
canal ~
g"'diSC} go
ampulla f
"- c:.
2...
Fig. 3. A schema of the inner structure of a sea urchin. From "The Invertebrates",
Stachowitsch, M., © 1992, Reprinted by permission of John Wiley & Sons Inc.
Results
Fig. 4. Culture of embryonic cells of the sea
urchin Strongylocentrotus purpuratus showing aggregation of migrated cells. x 150. From
Mintz et aI., 1981, with permission
After 10 h in culture, many cells form aggregates (Fig. 4). These aggregates later show spicules consisting of rod-like structures containing
CaC0 3 (Mintz et aI., 1981).
Subprotocol 3
Coelomocytes from Starfish (Asteroidea)
Materials
- Water bath
- Recirculating seawater system
- Hypodermic syringes (0.25-5.0 ml)
- Needles (25 or 26 G)
- Test tubes (15 ml, round bottomed)
- Pasteur pipettes
- No.2 round cover glass (22 mm diameter)
- Depression slide glass
Equipment