144
Methods for Setting Up Primary Cultures Specific to Animal Groups
c
o
c;,
!
OJ
. .
'" c
~
..
.c
'"
c
II
E
0
:& ..
(Ial eral )
. nleriof'
•
,
posl.rlo,
buccal siphon "'oral tentacle
sllgma
branchi al papilla
longitudinal bar
t.ansverse bar ---++HtI:/.::~
subendostylar
vessel
dlgesllve gland
pyloric cecum
heart
venlral _
dot.-I
dorsallanguet
ciliated funnel
anys
1 t"tHt-~.--- oviduct
vas deferens
muscle
esophagus
tunic
stomach
Fig.!. General structure of Tunicata. From "The Invertabates", Stachowitsch, M., ©
1992. Reprinted by permission of John Wiley & Sons, Inc.
z
Fig. 2. Schema of a colonial tunicata.
b, bud, s, stolon; z, zooid
3. Transfer the explants to the new wells of 24-well plates containing fresh
media every 7-11 days .
• • Comments
Tunicate plasma can be obtained as follows:
- Collect hemolymph from 100 S. clava into a chilled centrifuge tube
Methods for Setting Up Primary Cultures Specific to Animal Groups
c
o
c;,
!
OJ
. .
'" c
~
..
.c
'"
c
II
E
0
:& ..
(Ial eral )
. nleriof'
•
,
posl.rlo,
buccal siphon "'oral tentacle
sllgma
branchi al papilla
longitudinal bar
t.ansverse bar ---++HtI:/.::~
subendostylar
vessel
dlgesllve gland
pyloric cecum
heart
venlral _
dot.-I
dorsallanguet
ciliated funnel
anys
1 t"tHt-~.--- oviduct
vas deferens
muscle
esophagus
tunic
stomach
Fig.!. General structure of Tunicata. From "The Invertabates", Stachowitsch, M., ©
1992. Reprinted by permission of John Wiley & Sons, Inc.
z
Fig. 2. Schema of a colonial tunicata.
b, bud, s, stolon; z, zooid
3. Transfer the explants to the new wells of 24-well plates containing fresh
media every 7-11 days .
• • Comments
Tunicate plasma can be obtained as follows:
- Collect hemolymph from 100 S. clava into a chilled centrifuge tube