98
Methods for Setting Up Primary Cultures Specific to Animal Groups
• ill! Results
Fig. 6. Anatomy of the abdomen of the adult leafhopper (a layer of fat bodies has been removed).
mt, Malpighian tubules; my, mycetomes; ov,ovarioles; st, stomach
Fig. 7. Cell migration from adult
ovary (OV) of the six-spotted
leafhopper
Macrosteles
fascifrons. x 70
Cell migration may occur from the cut surface of the organs (Fig. 7). To
obtain sufficient migrated cells to make a subculture, a large number of
explants is required .
• ill! Comments
Organs that can be cultured using this method are usually the intestine
of aseptically reared insects, ovaries, testes, fat bodies, mycetomes, and
Malpighian tubes. A similar outgrowth of cells as that seen in embryonic
cell culture will be obtained.
Methods for Setting Up Primary Cultures Specific to Animal Groups
• ill! Results
Fig. 6. Anatomy of the abdomen of the adult leafhopper (a layer of fat bodies has been removed).
mt, Malpighian tubules; my, mycetomes; ov,ovarioles; st, stomach
Fig. 7. Cell migration from adult
ovary (OV) of the six-spotted
leafhopper
Macrosteles
fascifrons. x 70
Cell migration may occur from the cut surface of the organs (Fig. 7). To
obtain sufficient migrated cells to make a subculture, a large number of
explants is required .
• ill! Comments
Organs that can be cultured using this method are usually the intestine
of aseptically reared insects, ovaries, testes, fat bodies, mycetomes, and
Malpighian tubes. A similar outgrowth of cells as that seen in embryonic
cell culture will be obtained.