EtOH
Sterilization
~+
Dec.ntatlon II
Carlson's soln. .... Culture medium
~
....
........
o~
III
: :
Decantation II
Yolk
8 Insecta (Hemiptera)
93
.... ....
1\
0)
~~
Carlson's soln.
Fig. 1. Process of setting up leatbopper embryonic cell culture. EtOH, ethanol; so/n.,
solution
Fig. 2. Three types of cells that have migrated from the embryonic tissue of the rice
green leatbopper Nephotettix cincticeps. 1 Epithelial cell-like cells, 2 Fibroblast-like
cells, 3 Hemocyte-like cells. x 100
pers (Chiu and Black, 1967, 1969; Richardson and Jensen, 1971; Kimura,
1984). Homopteran insects other than leafboppers are extremely difficult to culture. Some migration has been obtained in cultures of embryonic tissues from the planthopper Laodelphax striatellus (Mitsuhashi,
1969; Yamada et aI., 1970).
Sterilization
~+
Dec.ntatlon II
Carlson's soln. .... Culture medium
~
....
........
o~
III
: :
Decantation II
Yolk
8 Insecta (Hemiptera)
93
.... ....
1\
0)
~~
Carlson's soln.
Fig. 1. Process of setting up leatbopper embryonic cell culture. EtOH, ethanol; so/n.,
solution
Fig. 2. Three types of cells that have migrated from the embryonic tissue of the rice
green leatbopper Nephotettix cincticeps. 1 Epithelial cell-like cells, 2 Fibroblast-like
cells, 3 Hemocyte-like cells. x 100
pers (Chiu and Black, 1967, 1969; Richardson and Jensen, 1971; Kimura,
1984). Homopteran insects other than leafboppers are extremely difficult to culture. Some migration has been obtained in cultures of embryonic tissues from the planthopper Laodelphax striatellus (Mitsuhashi,
1969; Yamada et aI., 1970).