Chapter 7
Insecta (Coleoptera)
Relatively few continuous cell lines have been established from Coleopteran insects. Culture media commonly used are modified IPL-52B,
Schneider's Drosophila medium, modified Grace's medium, and MGM450 medium (see Appendix O.
Subprotocol 1
Embryos
Materials
- Dissecting microscope
- Maximov slide
- Forceps
- Needles
- Knife
- Pasteur pipette
- Culture vessels
- Physiological solution
- Culture medium
- Eggs of coleopteran insects
Procedure
PROTOCOL
Equipment
Solutions
Animals
1. Surface sterilize eggs of beetles by submersion in 70% ethanol for 5 min. Sterilization
2. Wash the eggs with sterile distilled water.
1. Transfer the eggs into physiological solution placed in the depression Dissection
of a Maximov slide.
2. Cut the chorion of the eggs, and squeeze the content of the eggs out
under a dissecting microscope.
3. Remove the yolk surrounding the embryos using needles.
4. Collect the embryos, and wash them with fresh physiological solution.
5. Transfer the embryos into culture medium.