623
Isolation
Isolation from kidney was on TSA supplemented with 1.5 % (w/v) sea salts and 5 %
(v/v) defi brinated horse blood aerobically at 20 °C for 48 h when small grey colonies resulted. After initial isolation, culturing was possibly on TSA (or Columbia
agar) supplemented with 1.5 % (w/v) sodium chloride and 10 % (v/v) citrated sheep
or horse blood (Birkbeck et al. 2002 ).
Characteristics of the Pathogen
Four isolates were obtained (Birkbeck et al. 2002 ):
The phenotypic tests were used to link the pathogen to the Pasteurellaceae, and
the results of 16S rRNA sequencing confi rmed the association to the family with Pa.
phocoenarum (97.1 % homology) being regarded as closest neighbour
phylogenetically.
Epizootiology
The source of the pathogen may well have been fi sh, e.g. mackerel (Birkbeck et al.
2002 ).
Pathogenicity
I.p. injection of 1–4 × 10
6 cells led to some mortalities among experimental groups
of Atlantic salmon within 4 weeks at 15 °C (Birkbeck et al. 2002 ).
Box 11.13: Pasteurella skyensis
Cultures on blood-containing medium comprise non motile, facultatively
anaerobic pleomorphic Gram-negative catalase-negative rods that produce
esterase (lipase), indole, leucine arylamidase, lysine and ornithine decarboxylase, oxidase (weakly), acid and alkaline phosphatase and naphthol-ASBIphosphohydrolase but do not produce arginine dihydrolase, ß-galactosidase,
urease, the Voges Proskauer reaction or reduce nitrate. Acid is produced from
glucose, lactose, maltose, mannitol, mannose and trehalose, but not from
adonitol, arbutin, dulcitol, galactose, inositol, inulin, melibiose, raffi nose,
rhamnose, salicin, sucrose, sorbitol or xylose. Haemolytic activity is at best
weak. There is a requirement for blood and 1.5 % (w/v) for growth. Growth
occurs at 14–32 °C but not at 37 °C. The G + C ratio of the DNA is 39–41 mol%
(Birkbeck et al. 2002 ).
Pasteurellaceae Representative
Isolation
Isolation from kidney was on TSA supplemented with 1.5 % (w/v) sea salts and 5 %
(v/v) defi brinated horse blood aerobically at 20 °C for 48 h when small grey colonies resulted. After initial isolation, culturing was possibly on TSA (or Columbia
agar) supplemented with 1.5 % (w/v) sodium chloride and 10 % (v/v) citrated sheep
or horse blood (Birkbeck et al. 2002 ).
Characteristics of the Pathogen
Four isolates were obtained (Birkbeck et al. 2002 ):
The phenotypic tests were used to link the pathogen to the Pasteurellaceae, and
the results of 16S rRNA sequencing confi rmed the association to the family with Pa.
phocoenarum (97.1 % homology) being regarded as closest neighbour
phylogenetically.
Epizootiology
The source of the pathogen may well have been fi sh, e.g. mackerel (Birkbeck et al.
2002 ).
Pathogenicity
I.p. injection of 1–4 × 10
6 cells led to some mortalities among experimental groups
of Atlantic salmon within 4 weeks at 15 °C (Birkbeck et al. 2002 ).
Box 11.13: Pasteurella skyensis
Cultures on blood-containing medium comprise non motile, facultatively
anaerobic pleomorphic Gram-negative catalase-negative rods that produce
esterase (lipase), indole, leucine arylamidase, lysine and ornithine decarboxylase, oxidase (weakly), acid and alkaline phosphatase and naphthol-ASBIphosphohydrolase but do not produce arginine dihydrolase, ß-galactosidase,
urease, the Voges Proskauer reaction or reduce nitrate. Acid is produced from
glucose, lactose, maltose, mannitol, mannose and trehalose, but not from
adonitol, arbutin, dulcitol, galactose, inositol, inulin, melibiose, raffi nose,
rhamnose, salicin, sucrose, sorbitol or xylose. Haemolytic activity is at best
weak. There is a requirement for blood and 1.5 % (w/v) for growth. Growth
occurs at 14–32 °C but not at 37 °C. The G + C ratio of the DNA is 39–41 mol%
(Birkbeck et al. 2002 ).
Pasteurellaceae Representative