556
Isolation
Muscle from below the diseased skin was inoculated onto 5 % (v/v) sheep blood in
TSA and TCBS with incubation at 28 °C for 48 h (Zhang et al. 2014b ).
Characteristics of the Pathogen
Pathogenicity
Experimental challenge with yellow catfi sh confi rmed, pathogenicity with an LD 50
dose of 3.39 × 10
6 CFU/fi sh by i.p. injection when skin ulceration developed (Zhang
et al. 2014b ).
Disease Control
Vaccine OmpU has been recognized to be an important antigen, and upon i.p.
injection led to specifi c antibody production and protection against challenge.
OmpU must, therefore, be considered as possible candidate for an effective vaccine
(Cen et al. 2013 ).
Vibrio ordalii
Characteristics of the Disease
Disease caused by V. ordalii has been documented in Japan (e.g. Muroga et al. 1986 )
and the Pacifi c Northwest, USA. Essentially, the disease may be categorised as a
haemorrhagic septicaemia. However, there are subtle differences in the pathologies
of the diseases caused by V. anguillarum and V. ordalii. In the case of V. ordalii in
Box 10.12: Vibrio mimicus
Colonies are green on TCBS, and white on blood agar (ß-haemolytic).
Cultures contain Gram-negative curved rods, which grow in 0–6 % (w/v)
sodium chloride. Lysine decarboxylase and indole are produced, but not arginine dihydrolase or H 2 S. The Voges-Prokauer reaction is negative. Urea is not
attacked. Sequencing the 16S rRNA gene reveals 99.71 % homology with the
type strain of V. mimicus (and 99.42 % homology with the type strain of V.
cholerae) (Zhang et al. 2014b ).
10 Vibrios
Isolation
Muscle from below the diseased skin was inoculated onto 5 % (v/v) sheep blood in
TSA and TCBS with incubation at 28 °C for 48 h (Zhang et al. 2014b ).
Characteristics of the Pathogen
Pathogenicity
Experimental challenge with yellow catfi sh confi rmed, pathogenicity with an LD 50
dose of 3.39 × 10
6 CFU/fi sh by i.p. injection when skin ulceration developed (Zhang
et al. 2014b ).
Disease Control
Vaccine OmpU has been recognized to be an important antigen, and upon i.p.
injection led to specifi c antibody production and protection against challenge.
OmpU must, therefore, be considered as possible candidate for an effective vaccine
(Cen et al. 2013 ).
Vibrio ordalii
Characteristics of the Disease
Disease caused by V. ordalii has been documented in Japan (e.g. Muroga et al. 1986 )
and the Pacifi c Northwest, USA. Essentially, the disease may be categorised as a
haemorrhagic septicaemia. However, there are subtle differences in the pathologies
of the diseases caused by V. anguillarum and V. ordalii. In the case of V. ordalii in
Box 10.12: Vibrio mimicus
Colonies are green on TCBS, and white on blood agar (ß-haemolytic).
Cultures contain Gram-negative curved rods, which grow in 0–6 % (w/v)
sodium chloride. Lysine decarboxylase and indole are produced, but not arginine dihydrolase or H 2 S. The Voges-Prokauer reaction is negative. Urea is not
attacked. Sequencing the 16S rRNA gene reveals 99.71 % homology with the
type strain of V. mimicus (and 99.42 % homology with the type strain of V.
cholerae) (Zhang et al. 2014b ).
10 Vibrios