521
Isolation
This may be readily achieved from blood by inoculation onto TSA prepared with
seawater, TCBS, or seawater agar with incubation at 15–25 °C for 2–7 days. The
precise conditions employed by Colorni et al. ( 1981 ) were not stated. However, this
group succeeded in isolating V. alginolyticus, V. anguillarum and V. parahaemolyticus from blood, and long thin, fl exible rods from cases of gill rot. In addition, we
have isolated pure culture growth of V. alginolyticus from moribund eels. A selective and differential medium, termed Vibrio alginolyticus agar (VAL) has been
described, and which incorporates bile salts, high sodium chloride concentration
with a incubation at 37 °C (Appendix in Chap. 12 ). Using this approach, V. alginolyticus developed green-yellow colonies (Chang et al. 2011 ).
Characteristics of the Pathogen
Generally, fresh isolates matched the species description of V. alginolyticus
(Farmer et al. 2005 ).
Diagnosis
Molecular Methods A loop mediated isothermal amplifi cation PCR (LAMP) with
primers targeting the gyrB gene, was successful for the rapid and sensitive detection
[detection limit of 3.7 × 102 CFU/ml] of V. alginolyticus in diseased marine fi sh (Cai
et al. 2010 ).
Box 10.7: Vibrio alginolyticus
Typically, swarming growth develops on the surface of solid media. Cultures
comprise motile, fermentative Gram-negative rods that produce catalase, H 2 S,
indole, lysine and ornithine decarboxylase, and oxidase, but not arginine
dihydrolase or ß-galactosidase. Blood (haemolysis), chitin, gelatin, lipids,
starch and urea are degraded, but not aesculin. Nitrates are reduced. The
methyl red test and Voges Proskauer reaction are positive. Acid is produced
from glycerol, maltose, mannitol, mannose, salicin and sucrose, but not arabinose, inositol or lactose. Growth occurs at 37 °C, and in 7 % but not 0 % or
10 % (w/v) sodium chloride. Sensitivity is recorded to the vibriostatic agent,
O/129. The G + C ratio of the DNA is 45–47 moles%.
Vibrio alginolyticus
Isolation
This may be readily achieved from blood by inoculation onto TSA prepared with
seawater, TCBS, or seawater agar with incubation at 15–25 °C for 2–7 days. The
precise conditions employed by Colorni et al. ( 1981 ) were not stated. However, this
group succeeded in isolating V. alginolyticus, V. anguillarum and V. parahaemolyticus from blood, and long thin, fl exible rods from cases of gill rot. In addition, we
have isolated pure culture growth of V. alginolyticus from moribund eels. A selective and differential medium, termed Vibrio alginolyticus agar (VAL) has been
described, and which incorporates bile salts, high sodium chloride concentration
with a incubation at 37 °C (Appendix in Chap. 12 ). Using this approach, V. alginolyticus developed green-yellow colonies (Chang et al. 2011 ).
Characteristics of the Pathogen
Generally, fresh isolates matched the species description of V. alginolyticus
(Farmer et al. 2005 ).
Diagnosis
Molecular Methods A loop mediated isothermal amplifi cation PCR (LAMP) with
primers targeting the gyrB gene, was successful for the rapid and sensitive detection
[detection limit of 3.7 × 102 CFU/ml] of V. alginolyticus in diseased marine fi sh (Cai
et al. 2010 ).
Box 10.7: Vibrio alginolyticus
Typically, swarming growth develops on the surface of solid media. Cultures
comprise motile, fermentative Gram-negative rods that produce catalase, H 2 S,
indole, lysine and ornithine decarboxylase, and oxidase, but not arginine
dihydrolase or ß-galactosidase. Blood (haemolysis), chitin, gelatin, lipids,
starch and urea are degraded, but not aesculin. Nitrates are reduced. The
methyl red test and Voges Proskauer reaction are positive. Acid is produced
from glycerol, maltose, mannitol, mannose, salicin and sucrose, but not arabinose, inositol or lactose. Growth occurs at 37 °C, and in 7 % but not 0 % or
10 % (w/v) sodium chloride. Sensitivity is recorded to the vibriostatic agent,
O/129. The G + C ratio of the DNA is 45–47 moles%.
Vibrio alginolyticus