287
Disrupted cells/sub-cellular components
Virulent
Water-soluble
extract, toxoided
with alum
oral
Natural
Coho salmon
(juveniles)
Ambient −
ND
Klontz and
Anderson
(1970)
Virulent (AS-Sil
67 to AS SS 70)
LPS endotoxin i.p.
ND
Coho salmon
(juveniles)
7–18
ND
+ (1:10000)
Paterson and
Fryer ( 1974b )
NG
Toxoid
oral/i.p.
Natural
Coho salmon
(juveniles)
Ambient +/−
+ (1:16–1:2048)
Udey and Fryer
( 1978 )
B
Diluted with
SDS
h.i.
Natural
Brown trout
Ambient +/−
+ (1:40)
Smith et al.
(1980)
B
Dsrupted with
ultra-sonication
h.i.
Natural
Brown trout
Ambient +/−
ND
Smith et al.
(1980)
Virulent
Toxoid,
formalised
Oral
Natural
Brown trout (0+
years)
Ambient −
−
Austin and
Rodgers (1981)
Virulent
ECP, precipitated (NH 4 ) 2 SO 4
+ ethanol
i.p.
Water-borne
(juveniles)
Brook trout
12.5
+
+ (1:122)
Cipriano
( 1982a )
Virulent/avirulent Lysed broth
cult-ures + FCA
+ Al(OH) 3
i.p.
Immersion
Salmon
10–18
+
ND
McCarthy et al.
( 1983 )
Virulent
Toxoid,
formal-ised and
with chloroform
Oral
Natural
Rainbow trout
Ambient −
ND
Rodgers and
Austin (1985)
Virulent
ECP, precipit
ated with
(NH 4 ) 2 SO 4
i.p.
i.p.
Coho salmon
(juveniles)
13–15
+/−
+ (1:1280)
Olivier et al.
( 1985a )
Avirulent
As above
i.p.
i.p.
As above
13–15
−
+ (1:1280)
As above
Avirulent
Protease
i.m.
i.m.
Atlantic salmon
(0+ years)
NG
+
NG
Shieh ( 1985 )
(continued)
Aeromonas salmonicida
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