Table 1 Biohydrogen production from microalgae via photolysis
Microalgae
Process condition
Hydrogen
production
Reference
Anabaena
variabilis PK84
Indoor helical tubular
photobioreactor, Allen and
Arnon medium, air +2% CO 2 ,
12 h light and 12 h dark
cycles, 332 µE/m
2
/s
19.2 mL/h/
PhBR
Borodin et al.
(2000)
Anabaena
variabilis PK84
Outdoor tubular
photobioreactor, Allen and
Arnon medium, air +2% CO 2 ,
sunlight
45.8 mL/h/
PhBR
Tsygankov
et al. (2002)
Chlamydomonas
reinhardtii cc-124
Flat glass photobioreactor,
re-addition of sulphur (25 µM)
in TAP-S medium, argon
sparged, 300 µE/m
2
/s
5.94 µmol mg/
chl/h
Kosourov et al.
(2002)
Gloeocapsa
alpicola
Glass bottles, cells suspended
in Tris-HCl buffer, argon
sparged, 24 h dark
25 µL/h/mg d.
w
Troshina et al.
(2002)
Anabaena PCC
7120
Indoor tubular photobioreactor,
BG 11 0 medium, argon
sparged, 456 µE/m
2
/s
1.4 mL/h/
PhBR
Lindblad et al.
(2002)
Anabaena AMC
414
Indoor tubular photobioreactor,
BG 11 0 medium, argon
sparged, 456 µE/m
2
/s
13.8 mL/h/
PhBR
Lindblad et al.
(2002)
Immobilized
Chlamydomonas
reinhardtii
CC-1036 pf18 mt+
Flat plate photobioreactor,
TAP-S medium, argon
sparged, 120 µE/m
2
/s
6.4 µmol/mg
chl/h
Laurinavichene
et al. (2006)
Synechocystis
sp. PCC 6803
BG 11 medium with optimized
nutrients, nitrogen atmosphere,
dark condition
0.81 µmol/
mgchl/h
Burrows et al.
(2008)
Chlamydomonas
reinhardtii strain
L159I-N230Y
Flat plate photobioreactor,
TAP-S medium, 70 µmol/m
2
/s
5.77 mL/L/h
Torzillo et al.
(2009)
Synechocystis
sp. PCC 6803
Glass vial, BG11 0 -S medium,
750 mM b-mercaptoethanol,
argon sparged, 24 h dark
14.32 µmol/
mg chla/min
Baebprasert
et al. (2010)
Aphanothece
halophytica
Erlenmeyer flask,
nitrogen-deprived BG 11
medium, 30 µmol/m
2
/s for
18 h
13.8 µmol/
mgchl/h
Taikhao et al.
(2013)
Nostoc PCC 7120
DhupW
Flat panel photobioreactor, BG
11 medium, alternate argon/N 2
(20/80) and 100% argon
sparged, 44 lmol/m
2
/s
0.71 mmol/
mgchla/h
Nyberg et al.
(2015)
Lyngbya sp.
Glass reactors, medium
containing benzoate (600 mg/
L), argon sparged, 4000 lx
17.05 µmol/
gchl 1/h
Shi and Yu
(2016)
208
H. Singh and D. Das
Microalgae
Process condition
Hydrogen
production
Reference
Anabaena
variabilis PK84
Indoor helical tubular
photobioreactor, Allen and
Arnon medium, air +2% CO 2 ,
12 h light and 12 h dark
cycles, 332 µE/m
2
/s
19.2 mL/h/
PhBR
Borodin et al.
(2000)
Anabaena
variabilis PK84
Outdoor tubular
photobioreactor, Allen and
Arnon medium, air +2% CO 2 ,
sunlight
45.8 mL/h/
PhBR
Tsygankov
et al. (2002)
Chlamydomonas
reinhardtii cc-124
Flat glass photobioreactor,
re-addition of sulphur (25 µM)
in TAP-S medium, argon
sparged, 300 µE/m
2
/s
5.94 µmol mg/
chl/h
Kosourov et al.
(2002)
Gloeocapsa
alpicola
Glass bottles, cells suspended
in Tris-HCl buffer, argon
sparged, 24 h dark
25 µL/h/mg d.
w
Troshina et al.
(2002)
Anabaena PCC
7120
Indoor tubular photobioreactor,
BG 11 0 medium, argon
sparged, 456 µE/m
2
/s
1.4 mL/h/
PhBR
Lindblad et al.
(2002)
Anabaena AMC
414
Indoor tubular photobioreactor,
BG 11 0 medium, argon
sparged, 456 µE/m
2
/s
13.8 mL/h/
PhBR
Lindblad et al.
(2002)
Immobilized
Chlamydomonas
reinhardtii
CC-1036 pf18 mt+
Flat plate photobioreactor,
TAP-S medium, argon
sparged, 120 µE/m
2
/s
6.4 µmol/mg
chl/h
Laurinavichene
et al. (2006)
Synechocystis
sp. PCC 6803
BG 11 medium with optimized
nutrients, nitrogen atmosphere,
dark condition
0.81 µmol/
mgchl/h
Burrows et al.
(2008)
Chlamydomonas
reinhardtii strain
L159I-N230Y
Flat plate photobioreactor,
TAP-S medium, 70 µmol/m
2
/s
5.77 mL/L/h
Torzillo et al.
(2009)
Synechocystis
sp. PCC 6803
Glass vial, BG11 0 -S medium,
750 mM b-mercaptoethanol,
argon sparged, 24 h dark
14.32 µmol/
mg chla/min
Baebprasert
et al. (2010)
Aphanothece
halophytica
Erlenmeyer flask,
nitrogen-deprived BG 11
medium, 30 µmol/m
2
/s for
18 h
13.8 µmol/
mgchl/h
Taikhao et al.
(2013)
Nostoc PCC 7120
DhupW
Flat panel photobioreactor, BG
11 medium, alternate argon/N 2
(20/80) and 100% argon
sparged, 44 lmol/m
2
/s
0.71 mmol/
mgchla/h
Nyberg et al.
(2015)
Lyngbya sp.
Glass reactors, medium
containing benzoate (600 mg/
L), argon sparged, 4000 lx
17.05 µmol/
gchl 1/h
Shi and Yu
(2016)
208
H. Singh and D. Das