Part E | 28.3
704 Part E Marine Microbiology and Biotechnology
0
1
2
3
4
5
6
7
8
9
1 0
1 1
1 2
1 4
13
Time
Inten.
Time 14.337 Inten.
86 875
50 000
Time 9.030 Inten.
Time 10.978 Inten.
68 203
–85 118
Time 12.341 Inten.
15 min
µV (×10 000)
µV (×10 000)
Chromatogram
5
2.5
0
7.5
5
2.5
0
µV (×10 000)
5
2.5
0
µV (×10 000)
10
7.5
5
2.5
0
µV (×10 000)
a)
b)
c)
d)
e)
5
2.5
0
CN
CN
N
CN
N
OH
OH
O
OH
OH
O
N
O
NH 2
N
O
NH 2
Fig. 28.42a-e GC-FID Chromatograms. (a) Standard of 2-cyanopyridine 14. (b) Standard of phenylacetonitrile 1.
(c) Standard of 2-hydroxyphenylacetic acid 1a. (d) Standard of 2-pyridinecarboxamide 14a. (e) Biotransformation of 2cyanopyridine 14 (20:0 L, 0:21 mmol) by A. sydowii CBMAI 934 in 96 h. Conditions of GC-FID analysis: T i D 100
ı C
(2 min), T f D 160
ı C (2 min), r D 5
ı C=min, t c D 16 min
TIC
Intensity
201 028
4
1 0
8553
8821
20
30 min
200 000
150000
100000
50000
0
OH
OH
O
N
O
NH 2
b)
a)
Fig. 28.43a,b GC-MS chromatogram. (a) Peak corresponding to biotransformation of phenylacetonitrile 1 to 2hydroxyphenylacetic acid 1a by Aspergillus sydowii CBMAI 934 in 96 h. (b) Peak corresponding to biotransformation
of 2-cyanopyridine 14 (20:0 L, 0:21 mmol) to 2-pyridinecarboxamide 14a by Aspergillus sydowii CDMAI 934 in 96 h