Microfluidic Systems for Marine Biotechnology 20.2 Microfluidic Devices for Marine Biology and Ecosystem Studies 513
Part C | 20.2
a)
b)
c)
d)
A
D
B
E
F
C
B
B
C
E
D
F
A
F
C
G
D
H
E
5 mm
I
Neutralization buffer
loading
Neutralization reaction
Feed line washing
Reaction mix loading
Amplification reaction
Cell in chamber
Lysis buffer in feed line
Lysis buffer loading
Aqueous segment
Air bubble
Carrier liquid
Age 6 h
Embryo
Age 72 h
Larvae
Lysis reaction
Feed line washing
impedance, measured at different frequencies, characterized the cells in function of their size and the
capacitance of their membrane. The results obtained by
this microfluidic flow cytometer were comparable with
those obtained with a commercial flow cytometer, and
the low-frequency electrical impedance signal used to
size the particles was analogous to the forward scatter
signal obtained in a flow cytometer. However, the mi-
Part C | 20.2
a)
b)
c)
d)
A
D
B
E
F
C
B
B
C
E
D
F
A
F
C
G
D
H
E
5 mm
I
Neutralization buffer
loading
Neutralization reaction
Feed line washing
Reaction mix loading
Amplification reaction
Cell in chamber
Lysis buffer in feed line
Lysis buffer loading
Aqueous segment
Air bubble
Carrier liquid
Age 6 h
Embryo
Age 72 h
Larvae
Lysis reaction
Feed line washing
impedance, measured at different frequencies, characterized the cells in function of their size and the
capacitance of their membrane. The results obtained by
this microfluidic flow cytometer were comparable with
those obtained with a commercial flow cytometer, and
the low-frequency electrical impedance signal used to
size the particles was analogous to the forward scatter
signal obtained in a flow cytometer. However, the mi-