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Y. Kumazawa et al.
Table 1. Scientific names and current classification of taxa sequenced in this
study
Species
African cichlids
Tylochromis polylepis
Oreoehromis nilotieus
Tropheus moorii
South American cichlids
Ciehlasoma eitrinellum
Geophagus jurupari
Astronotus ocellatus
others
Ditrema temmilleki
Chrysiptera cyanea
Pselldolabrus japonieus
Traclwrus japonicus
Apogoll semilineatus
Scomber japonieus
Rhinogobius giurinlls
Phellacogrammus interruptus
Amia calva
Classification'
Cichlidae, Labroidei, Perciformes
Cichlidae, Labroidei, Perciformes
Cichlidae, Labroidei, Perciformes
Cichlidae, Labroidei, Perciformes
Cichlidae, Labroidei, Perciformes
Cichlidae, Labroidei, Perciformes
Embiotocidae, Labroidci, Perciformes
Pomacentridae, Labroidei, Perciformes
Labridae, Labroidei, Perciformes
Carangidae, Percoidei, Perciformes
Apogonidae, Percoidei, Perciformes
Scombridae, Scombroidei, Perciformes
Gobiidae, Gobioidei, Perciformes
Characidae, - - - , Characiformes
Amiidae, - - - , Amiiformes
Although complete ND2 gene sequences were reported for T. polylepis, O.
niloticus, T. moorii, and C. eitrinellum (Kocher et al. 1995), we determined both
ND2 and cytb gene sequences for these species using our specimens.
"Classification is based on Nelson (1994). Names for the family, suborder, and
order are shown from left to right. No suborder name is given to P. interruptus
and A. calva.
55°C for 1 min, and extension at 72°C for 1-3 min. Amplified products were purified with QIAquick PCR purification kit (QIAGEN) and used directly as template
for sequence determination with the Applied Biosystems 373A DNA Sequencer.
Complete nucleotide sequences for the ND2 and cytb genes were determined unambiguously by sequencing both strands from internal sequencing primers listed in
Table 2 (or some taxon-specific internal primers, data not shown) as well as those
used for the initial amplification. Database accession numbers for the sequences
determined in this study are AB018970-AB018999.