-3.5kb
~
~.
_ _ _ _ _
J~
~~~--~
I· Ci-tkh
upstream
PfBra
Ci-fkh
uP~~~~.!!1 .
. 1 Ci-Bra
Fig.
3. Formation of extra notochords by an ectopic expression of chordate and non-chordate deuterostome Brachyury
genes. (A) Induction of extra
notochord (arrow) by an ectopic expression of the ascidian Ci-Bra
with the aid of Ci-fkh/Ci-Bra vector electroporation. Formation of notochord was
visualized by whole-mount in situ
hybridization with a notochord-specific gene probe. White asterisks indicate the notochord formed normally by CiBra
expression (A-C). (B)
Induction of extra notochords by an ectopic expression ofthe amphioxus Am(Bb)Bra2
with the aid of Ci-fkh!Arn(Bb)Bra2
vector electroporation. (C) Induction of extra notochords by an ectopic expression of the acorn worm PfBra
with the aid of Ci-fkh/PfBra vector
electroporation
.....
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