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FISH Analysis of Oligotrophic Bacteria Distribution in Marine Environments
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Table 1 • .probe designed from bacterial16S rDNA sequences that were retrieved from the highest positive dilutions of seawater sample from 30 m of depth (station DYFAMED)
Clone
EMBL
Nearest relative in EMBL database
Probe sequence
rRNA
accession no.
(% similarity)
(5'-3')
position a
SRFI
AJ002563
Roseobacter algocolus (95.0)
GTCAGTATCGAGCCAGTGAG
645-626
SRF2
AJ002564
Roseobacter algocolus (95.0)
GTCAGTATCGAGCCAGTGAG
645-626
SRF3
AJOO2565
Silicibacter lacustaerulensis (94.7)
GTCAGTATCGAGCCAGTGAG
645-626
CTCAAGACTACCAGTATTAG
806-787
DPT1.l
AJOO2566
Vibrio sp.2P44 (98.0)
AAATCCTCCGAAGATTCAAT
47-28
AGGCCACAACCTCCAAGTAG
841-822
DPT1.2
AJ002567
Vibrio sp.2P44 (98.0)
AAATCCTCCGAAGATTCAAT
47-28
AGGCCACAACCTCCAAGTAG
841-822
DPT1.3
AJOO2569
Plavobacter salegens (87.5)
GGTTCTTCCTCTGTAAAAGC
502-483
CTGGCAACTAACCACAGGGG
1128-1109
DPT1.4
AJOO2570
Plavobacter salegens (87.3)
GGTTCTTCCTCTGTAAAAGC
502-483
CTGGCAACTAACCACAGGGG
1128-1109
DPT2.1
AJOO2568
Vibrio sp.2P44 (93.0)
AAATCCTCCGAAGATTCAAT
47-28
AGGCCACAACCTCCAAGTAG
841-822
a Escherichia coli numbering
Fig. la,b. a DAPI stained cells and b cells hybridized with CY3-G Rb probe from natural microbial community of 30 m depth
sample under epifluorescence microscopy examination
Fig.2a,h. a DAF! stained cells and b cells hybridized with CY3-G V probe from natural microbial community of 30 m depth sample under epifluorescence microscopy examination
b
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