147
H.-J. Ceccaldi et al. (eds.), Marine Productivity: Perturbations and Resilience of Socio-ecosystems,
DOI 10.1007/978-3-319-13878-7_16, © Springer International Publishing Switzerland 2015
Abstract
Growth in fi sh is regulated by the growth hormone (GH)-growth hormone
receptor (GHR)-insulin-like growth factor-1 (IGF-1) axis. However, the
effect of severe acute stressors on the GH-IGF-1 axis in fi sh is not well
understood. The present study determined the changes in mRNA expression of growth-related genes gh , ghr , and igf and the redox state in coho
salmon ( Oncorhynchus kisutch ), in response to severe acute stress.
Severe stress consisted of exposure to heat shock (adequate rearing temperature +11 °C for 2 h). The plasma expression patterns of redox staterelated biomarkers, such as glutathione, lipid peroxides, and superoxide
dismutase, in response to heat shock suggest that heat shock might
induce oxidative stress in fi sh. After exposure to heat shock, ghr mRNA
levels in the pituitary glands and liver increased, whereas levels decreased
48 h post- stress. Hepatic igf1 mRNA expression levels gradually
decreased in response to the stressor. On the other hand, the pituitary gh
mRNA expression did not change in response to the stressor. These fi ndings showed that a heat shock-induced oxidative stress could affect the
redox state and the expression of several growth-related genes in coho
salmon. The results of this study also suggest that the expression of several growth-related genes in fi sh may be affected differently by the types
and strength of stress.
T. Nakano (*) • M. Kameda
T. Yamaguchi • M. Sato
Marine Biochemistry Laboratory, Graduate School of
Agricultural Science , Tohoku University ,
Aoba-ku, Sendai 981-8555 , Japan
e-mail: nakanot@bios.tohoku.ac.jp
L. O. B. Afonso
School of Life and Environmental Sciences, Faculty
of Science and Technology , Deakin University ,
Warrnambool , VIC 3280 , Australia
Effects of Thermal Stressors
on Growth-Related Gene
Expressions in Cultured Fish
Toshiki Nakano , Masumi Kameda ,
Toshiyasu Yamaguchi , Minoru Sato ,
Luis O. B. Afonso , George K. Iwama ,
and Robert H. Devlin
G. K. Iwama
Okinawa Institute of Science and Technology,
Onna-Son, Kunigami, Okinawa 904-0495 ,
Japan
R. H. Devlin
West Vancouver Laboratory, Center for Aquaculture
and Environment Research (CAER- DFO/UBC) ,
Fisheries and Oceans Canada , West Vancouver ,
BC V7V 1N6 , Canada
H.-J. Ceccaldi et al. (eds.), Marine Productivity: Perturbations and Resilience of Socio-ecosystems,
DOI 10.1007/978-3-319-13878-7_16, © Springer International Publishing Switzerland 2015
Abstract
Growth in fi sh is regulated by the growth hormone (GH)-growth hormone
receptor (GHR)-insulin-like growth factor-1 (IGF-1) axis. However, the
effect of severe acute stressors on the GH-IGF-1 axis in fi sh is not well
understood. The present study determined the changes in mRNA expression of growth-related genes gh , ghr , and igf and the redox state in coho
salmon ( Oncorhynchus kisutch ), in response to severe acute stress.
Severe stress consisted of exposure to heat shock (adequate rearing temperature +11 °C for 2 h). The plasma expression patterns of redox staterelated biomarkers, such as glutathione, lipid peroxides, and superoxide
dismutase, in response to heat shock suggest that heat shock might
induce oxidative stress in fi sh. After exposure to heat shock, ghr mRNA
levels in the pituitary glands and liver increased, whereas levels decreased
48 h post- stress. Hepatic igf1 mRNA expression levels gradually
decreased in response to the stressor. On the other hand, the pituitary gh
mRNA expression did not change in response to the stressor. These fi ndings showed that a heat shock-induced oxidative stress could affect the
redox state and the expression of several growth-related genes in coho
salmon. The results of this study also suggest that the expression of several growth-related genes in fi sh may be affected differently by the types
and strength of stress.
T. Nakano (*) • M. Kameda
T. Yamaguchi • M. Sato
Marine Biochemistry Laboratory, Graduate School of
Agricultural Science , Tohoku University ,
Aoba-ku, Sendai 981-8555 , Japan
e-mail: nakanot@bios.tohoku.ac.jp
L. O. B. Afonso
School of Life and Environmental Sciences, Faculty
of Science and Technology , Deakin University ,
Warrnambool , VIC 3280 , Australia
Effects of Thermal Stressors
on Growth-Related Gene
Expressions in Cultured Fish
Toshiki Nakano , Masumi Kameda ,
Toshiyasu Yamaguchi , Minoru Sato ,
Luis O. B. Afonso , George K. Iwama ,
and Robert H. Devlin
G. K. Iwama
Okinawa Institute of Science and Technology,
Onna-Son, Kunigami, Okinawa 904-0495 ,
Japan
R. H. Devlin
West Vancouver Laboratory, Center for Aquaculture
and Environment Research (CAER- DFO/UBC) ,
Fisheries and Oceans Canada , West Vancouver ,
BC V7V 1N6 , Canada