120
This instrument is designed to operate autonomous
in situ monitoring of a wide number of phytoplanktonic species in the range of size between 1
and 800 µm (Dubelaar et al. 1999 ). Analyses
are made at the single cell level and at high
frequency (up to once every 10 min) (Dubelaar
and Gerritzen 2000 ; Peeters et al. 1989 ). This
instrument has been used to reveal the temporal
and spatial phytoplankton variability in situ
(Thyssen et al. 2008 ). Autotrophic cells are triggered on the basis of their chlorophyll a content,
which naturally emits red fl uorescence. When the
cells pass through the light source excitation
(a 488 nm laser beam), fi ve optical profi les
Fig. 1 Surface chlorophyll a
concentration measured at
the 10 hydrological stations
in the Berre lagoon for the
sampling performed in
September 2011
Cytosense
N
laboratory
(sampling point)
100 m
5.166
43.469
43.470
43.471
43.472
43.473
5.168
5.170
5.172
5.174
Fig. 2 Location of the
sampling point ( black
circle ) and the 250 m pipe
( black line ) until the
laboratory, on the “Berre
l’Etang” harbor. Flow
cytometry analyses were
automatically run every
hour by the Cytosense fl ow
cytometer. When needed,
the cytometer could be
remotely operated thanks
to an Internet connection
M. Dugenne et al.
This instrument is designed to operate autonomous
in situ monitoring of a wide number of phytoplanktonic species in the range of size between 1
and 800 µm (Dubelaar et al. 1999 ). Analyses
are made at the single cell level and at high
frequency (up to once every 10 min) (Dubelaar
and Gerritzen 2000 ; Peeters et al. 1989 ). This
instrument has been used to reveal the temporal
and spatial phytoplankton variability in situ
(Thyssen et al. 2008 ). Autotrophic cells are triggered on the basis of their chlorophyll a content,
which naturally emits red fl uorescence. When the
cells pass through the light source excitation
(a 488 nm laser beam), fi ve optical profi les
Fig. 1 Surface chlorophyll a
concentration measured at
the 10 hydrological stations
in the Berre lagoon for the
sampling performed in
September 2011
Cytosense
N
laboratory
(sampling point)
100 m
5.166
43.469
43.470
43.471
43.472
43.473
5.168
5.170
5.172
5.174
Fig. 2 Location of the
sampling point ( black
circle ) and the 250 m pipe
( black line ) until the
laboratory, on the “Berre
l’Etang” harbor. Flow
cytometry analyses were
automatically run every
hour by the Cytosense fl ow
cytometer. When needed,
the cytometer could be
remotely operated thanks
to an Internet connection
M. Dugenne et al.