117
H.-J. Ceccaldi et al. (eds.), Marine Productivity: Perturbations and Resilience of Socio-ecosystems,
DOI 10.1007/978-3-319-13878-7_13, © Springer International Publishing Switzerland 2015
Abstract
The vicinity of urban activity and industry (petrochemistry) around the
Berre lagoon (southeast of France) has induced the degradation of its
ecosystem, characterized by a permanent eutrophic state. In particular, a
power plant has discharged substantial inputs of enriched freshwater in
the lagoon since 1966. Due to these high nutrient inputs and also to
regeneration rates, several species of phytoplankton regularly bloom in the
lagoon at spring, summer, or autumn. Peaks of phytoplanktonic biomass
(>150 µg Chl a /dm
3 ) are generally followed by intense heterotrophic activities leading to O 2 consumption with hypoxic or anoxic episodes. The
study of phytoplankton dynamics is thus of primary importance.
Within the framework of an ecological survey, an automated platform
of in situ instruments was set up in a laboratory located on the “Berre
l’Etang” harbor to assess biological and hydrological features of the lagoon
at short time scale during October 2011. The phytoplanktonic community
was characterized by a Cytosense autonomous fl ow cytometer (Cytobuoy,
Netherlands) operating at high frequency (hourly sampling) in order
to detect sudden changes of species compositions and abundances. In
parallel, hydrological sensors have measured several physicochemical
variables of the water directly pumped from the lagoon (1.5 m depth) to
the fi eld laboratory.
In October 2011, among the various phytoplanktonic species optically
resolved by the Cytosense, the dinofl agellate Akashiwo sanguinea has
M. Dugenne • M. Thyssen
N. Garcia • G. Grégori (*)
Université Sud Toulon Var, IRD, CNRS ,
Aix- Marseille University , Marseille , France
Mediterranean Institute of Oceanography ,
Marseille , France
e-mail: gerald.gregori@mio.osupytheas.fr
Monitoring of a Potential Harmful
Algal Species in the Berre Lagoon
by Automated In Situ Flow
Cytometry
Mathilde Dugenne , Mélilotus Thyssen ,
Nicole Garcia , Nicolas Mayot , Guillaume Bernard ,
and Gérald Grégori
N. Mayot • G. Bernard
GIPREB Syndicat Mixte , Berre-l’Étang , France
H.-J. Ceccaldi et al. (eds.), Marine Productivity: Perturbations and Resilience of Socio-ecosystems,
DOI 10.1007/978-3-319-13878-7_13, © Springer International Publishing Switzerland 2015
Abstract
The vicinity of urban activity and industry (petrochemistry) around the
Berre lagoon (southeast of France) has induced the degradation of its
ecosystem, characterized by a permanent eutrophic state. In particular, a
power plant has discharged substantial inputs of enriched freshwater in
the lagoon since 1966. Due to these high nutrient inputs and also to
regeneration rates, several species of phytoplankton regularly bloom in the
lagoon at spring, summer, or autumn. Peaks of phytoplanktonic biomass
(>150 µg Chl a /dm
3 ) are generally followed by intense heterotrophic activities leading to O 2 consumption with hypoxic or anoxic episodes. The
study of phytoplankton dynamics is thus of primary importance.
Within the framework of an ecological survey, an automated platform
of in situ instruments was set up in a laboratory located on the “Berre
l’Etang” harbor to assess biological and hydrological features of the lagoon
at short time scale during October 2011. The phytoplanktonic community
was characterized by a Cytosense autonomous fl ow cytometer (Cytobuoy,
Netherlands) operating at high frequency (hourly sampling) in order
to detect sudden changes of species compositions and abundances. In
parallel, hydrological sensors have measured several physicochemical
variables of the water directly pumped from the lagoon (1.5 m depth) to
the fi eld laboratory.
In October 2011, among the various phytoplanktonic species optically
resolved by the Cytosense, the dinofl agellate Akashiwo sanguinea has
M. Dugenne • M. Thyssen
N. Garcia • G. Grégori (*)
Université Sud Toulon Var, IRD, CNRS ,
Aix- Marseille University , Marseille , France
Mediterranean Institute of Oceanography ,
Marseille , France
e-mail: gerald.gregori@mio.osupytheas.fr
Monitoring of a Potential Harmful
Algal Species in the Berre Lagoon
by Automated In Situ Flow
Cytometry
Mathilde Dugenne , Mélilotus Thyssen ,
Nicole Garcia , Nicolas Mayot , Guillaume Bernard ,
and Gérald Grégori
N. Mayot • G. Bernard
GIPREB Syndicat Mixte , Berre-l’Étang , France