79
H.-J. Ceccaldi et al. (eds.), Marine Productivity: Perturbations and Resilience of Socio-ecosystems,
DOI 10.1007/978-3-319-13878-7_9, © Springer International Publishing Switzerland 2015
Abstract
The aim of this study based on a multidisciplinary research program
(FLASH) (FLASH (FLuxes of Antibiotic and Antibiotic- resistant bacteria
and the corresponding genes in Surface Hydrosystems) a research project
founded by CNRS EC2CO/GIP Seine-Aval), associating chemists,
hydrologists, and clinical and environmental microbiologists, was to
monitor the impact of antibiotic prescription in human and veterinary practices on water and sediment contamination by antibiotic and Escherichia
coli (antibiotic resistance, integrons) and Enterococci (diversity, antibiotic
resistance, and the corresponding genes) along a medical center–WWTP–
river continuum. For this purpose, a multi- residue chemical methodology
was developed in order to detect low levels of 34 antibiotics. In the
medical center, the main prescribed antibiotic (amoxicillin) was weakly
found in effl uents. Along the continuum, contamination of water by
antibiotics decreased from 160 µg.L
−1 (cefotaxime) in hospital effl uents
to 1 ng.L
−1 (ofl oxacin) in the river. These concentrations were too low
to exert a selective pressure (mg.L
−1
) on antibiotic-resistant bacteria.
F. Petit (*) • J. Deloffre • K. Oberlé • T. Berthe
UMR CNRS M2C, SFR SCALE ,
Normandie Université , Rouen , France
e-mail: fabienne.petit@univ-rouen.fr
E. Denamur • O. Clermont
INSERM U1137 IAME , Université Paris Diderot ,
Sorbonne Paris Cité , France
Fate of Antibiotics and AntibioticResistant Fecal Bacteria
in Water and Sediments
from the Contamination Source
to the Estuary: Impact and/or
Resilience? Resilience
to Contamination by Antibiotics
Fabienne Petit , Erick Denamur , Olivier Clermont ,
Roland Leclercq , Julien Deloffre , Vincent Cattoir ,
Kenny Oberlé , Hélène Budzinski , and Thierry Berthe
R. Leclercq • V. Cattoir
EA4655, CHU de Caen , Université de Caen Basse ,
Normandie Caen , France
H. Budzinski
UMR CNRS 5805 EPOC-LPTC ,
Université de Bordeaux , Talence , France
H.-J. Ceccaldi et al. (eds.), Marine Productivity: Perturbations and Resilience of Socio-ecosystems,
DOI 10.1007/978-3-319-13878-7_9, © Springer International Publishing Switzerland 2015
Abstract
The aim of this study based on a multidisciplinary research program
(FLASH) (FLASH (FLuxes of Antibiotic and Antibiotic- resistant bacteria
and the corresponding genes in Surface Hydrosystems) a research project
founded by CNRS EC2CO/GIP Seine-Aval), associating chemists,
hydrologists, and clinical and environmental microbiologists, was to
monitor the impact of antibiotic prescription in human and veterinary practices on water and sediment contamination by antibiotic and Escherichia
coli (antibiotic resistance, integrons) and Enterococci (diversity, antibiotic
resistance, and the corresponding genes) along a medical center–WWTP–
river continuum. For this purpose, a multi- residue chemical methodology
was developed in order to detect low levels of 34 antibiotics. In the
medical center, the main prescribed antibiotic (amoxicillin) was weakly
found in effl uents. Along the continuum, contamination of water by
antibiotics decreased from 160 µg.L
−1 (cefotaxime) in hospital effl uents
to 1 ng.L
−1 (ofl oxacin) in the river. These concentrations were too low
to exert a selective pressure (mg.L
−1
) on antibiotic-resistant bacteria.
F. Petit (*) • J. Deloffre • K. Oberlé • T. Berthe
UMR CNRS M2C, SFR SCALE ,
Normandie Université , Rouen , France
e-mail: fabienne.petit@univ-rouen.fr
E. Denamur • O. Clermont
INSERM U1137 IAME , Université Paris Diderot ,
Sorbonne Paris Cité , France
Fate of Antibiotics and AntibioticResistant Fecal Bacteria
in Water and Sediments
from the Contamination Source
to the Estuary: Impact and/or
Resilience? Resilience
to Contamination by Antibiotics
Fabienne Petit , Erick Denamur , Olivier Clermont ,
Roland Leclercq , Julien Deloffre , Vincent Cattoir ,
Kenny Oberlé , Hélène Budzinski , and Thierry Berthe
R. Leclercq • V. Cattoir
EA4655, CHU de Caen , Université de Caen Basse ,
Normandie Caen , France
H. Budzinski
UMR CNRS 5805 EPOC-LPTC ,
Université de Bordeaux , Talence , France