118
J.-N. Chen and M. C. Fishman
et al. 1999; Hukriede et al. 1999). Hundreds of SSLP markers have been
typed on both panels as anchors. Mapping large number of ESTs and
cDNAs on the RH panel will accelerate the molecular analysis of mutations by providing large pool of candidates. Furthermore, mapping large
number of genes will provide the means to compare syntenic relationships between species.
7.7 Conclusion
In the past, molecular analysis of organ formation was limited to the
study of known genes or pathways, but an application of the logical
steps of organogenesis cannot be achieved so. Zebrafish offer a new
opportunity to apply genetics in combination with embryology. The first
screens show that single steps can be revealed genetically for organ
formation, in a manner akin to those for body formation in Drosophila.
For example, the genetic steps for chamber formation and patterning,
and for left-right laterality are made evident by mutational analysis.
Each such gene provides a molecular handle to pathways, other components of which may be discovered by biochemical or genetic techniques.
Other organs, including the kidney, gut and pancreas, are proving receptive to this approach as well.
References
Azpiazu N, Frasch M (1993) tinman and bagpipe: two homeo box genes that
determine cell fates in the dorsal mesoderm of Drosophila. Genes Dev
7:1325-1340
Bauer H, Meier A, Hild M, Stachel S, Economides A, Hazelett D, Harland
RM, Hammerschmidt M (1998) Follistatin and noggin are excluded from
the zebrafish organizer. Dev Bioi 204:488-507
Bodmer R (1993) The gene tinman is required for specification of the heart
and visceral muscles in Drosophila. Development 118:719-729
Brownlie A, Donovan A, Pratt SJ, Paw BH, Oates AC, Brugnara C, Witkowska
HE, Sassa S, Zon LI (1998) Positional cloning of the zebrafish sauternes
gene: a model for congenital sideroblastic anaemia. Nat Genet 20:244-250
Chen J-N, Fishman MC (1997) Development of Cardiovascular System: Molecules to Organisms. Cambridge University Press, Cambridge
J.-N. Chen and M. C. Fishman
et al. 1999; Hukriede et al. 1999). Hundreds of SSLP markers have been
typed on both panels as anchors. Mapping large number of ESTs and
cDNAs on the RH panel will accelerate the molecular analysis of mutations by providing large pool of candidates. Furthermore, mapping large
number of genes will provide the means to compare syntenic relationships between species.
7.7 Conclusion
In the past, molecular analysis of organ formation was limited to the
study of known genes or pathways, but an application of the logical
steps of organogenesis cannot be achieved so. Zebrafish offer a new
opportunity to apply genetics in combination with embryology. The first
screens show that single steps can be revealed genetically for organ
formation, in a manner akin to those for body formation in Drosophila.
For example, the genetic steps for chamber formation and patterning,
and for left-right laterality are made evident by mutational analysis.
Each such gene provides a molecular handle to pathways, other components of which may be discovered by biochemical or genetic techniques.
Other organs, including the kidney, gut and pancreas, are proving receptive to this approach as well.
References
Azpiazu N, Frasch M (1993) tinman and bagpipe: two homeo box genes that
determine cell fates in the dorsal mesoderm of Drosophila. Genes Dev
7:1325-1340
Bauer H, Meier A, Hild M, Stachel S, Economides A, Hazelett D, Harland
RM, Hammerschmidt M (1998) Follistatin and noggin are excluded from
the zebrafish organizer. Dev Bioi 204:488-507
Bodmer R (1993) The gene tinman is required for specification of the heart
and visceral muscles in Drosophila. Development 118:719-729
Brownlie A, Donovan A, Pratt SJ, Paw BH, Oates AC, Brugnara C, Witkowska
HE, Sassa S, Zon LI (1998) Positional cloning of the zebrafish sauternes
gene: a model for congenital sideroblastic anaemia. Nat Genet 20:244-250
Chen J-N, Fishman MC (1997) Development of Cardiovascular System: Molecules to Organisms. Cambridge University Press, Cambridge