127
Murray N, Bruce S, Murray K (1979) Molecular cloning of DNA ligase gene from bacteriophage
T4. II. Amplification and preparation of the gene product. J Mol Biol 132:493
Myers TW, Gelfand DH (1991) Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 30(31):7661–7666
Nossal NG (1984) Prokaryotic DNA replication systems. Annu Rev Biochem 53:581–615
Panasenko SM, Alazard RJ, Lehman IR (1978) A simple, three-step procedure for the large
scale purification of DNA ligase from a hybrid λ lysogen constructed in vitro. J Biol Chem
253:4590–4592
Perbal B (2008) Avian myeoloblastosis virus (AMV): only one side of the coin. Retrovirology 5:49
Pray L (2008) Restriction enzymes. Nat Educ 1(1):38
Raines RT (1998) Ribonuclease A. Chem Rev 98(3):1045–1066
Rittié L, Perbal B (2008) Enzymes used in molecular biology: a useful guide. J Cell Commun
Signal 2(1–2):25–45
Rogers SG, Weiss B (1980) Cloning of the exonuclease III gene of Escherichia coli. Gene
11:187–195
Saïda F, Odaert B, Uzan M, Bontems F (2004) First structural investigation of the restriction
ribonuclease RegB: NMR spectroscopic conditions, 13C/15N double-isotopic labelling and
two-dimensional heteronuclear spectra. Protein Expr Purif 34(1):158–165
Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988)
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
Science 239(4839):487–491
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc
Natl Acad Sci U S A 74:5463–5467
Stahl SJ, Zinn K (1981) Nucleotide sequence of the cloned gene for bacteriophage T7 RNA polymerase. J Mol Biol 148(4):481–485
Stein H, Hausen P (1969) Enzyme from calf thymus degrading the RNA moiety of DNA-RNA
hybrids: effect on DNA-dependent RNA polymerase. Science 166:393–395
Steitz TA (1998) A mechanism for all polymerases. Nature 391:231–232
Stenesh J, Roe B (1972) DNA polymerase from mesophilic and thermophilic bacteria. I. Purification
and properties from Bacillus licheniformis and Bacillus stearothermophilus. Biochim Biophys
Acta 272:167
Struhl K (1997) Enzymatic manipulation of DNA and RNA. In: Bensonchanda V (ed) Current
protocols in molecular biology. Wiley, Boston, pp 311–316
Tabor S, Richardson CC (1989) Selective inactivation of the exonuclease activity of bacteriophage
T7 DNA polymerase by in vitro mutagenesis. J Biol Chem 264:6447–6458
Tabor S, Huber HE, Richardson CC (1987) Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. J Biol Chem
262:16212–16223
Takagi M, Nishioka M, Kakihara H, Kitabayashi M, Inoue H, Kawakami B, Imanaka T (1997)
Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to
PCR. Appl Environ Microbiol 63(11):4504–4510
Takahashi K (1966) The structure and function of ribonuclease T1. VII. Further investigations
on amino acid composition and some other properties of ribonuclease T1. J Biochem 60:239
Talmadge K, Stahl S, Gilbert W (1980) Eukaryotic signal sequence transports insulin antigen in
Escherichia coli. Proc Natl Acad Sci U S A 77:3369–3373
Vanecko S, Laskowski M (1961) Studies of the specificity of deoxyribonuclease I. III. Hydrolysis
of chains carrying a monoesterified phosphate on carbon 5. J Biol Chem 236:3312–3316
Verjee ZHM (1969) Isolation of three acid phosphatases from wheat germ. Eur J Biochem
9(3):439–444
Wang LK, Lima CD, Shuman S (2002) Structure and mechanism of T4 polynucleotide kinase: an
RNA repair enzyme. EMBO J 21(14):3873–3880
4 Enzymes as Molecular Tools
Murray N, Bruce S, Murray K (1979) Molecular cloning of DNA ligase gene from bacteriophage
T4. II. Amplification and preparation of the gene product. J Mol Biol 132:493
Myers TW, Gelfand DH (1991) Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 30(31):7661–7666
Nossal NG (1984) Prokaryotic DNA replication systems. Annu Rev Biochem 53:581–615
Panasenko SM, Alazard RJ, Lehman IR (1978) A simple, three-step procedure for the large
scale purification of DNA ligase from a hybrid λ lysogen constructed in vitro. J Biol Chem
253:4590–4592
Perbal B (2008) Avian myeoloblastosis virus (AMV): only one side of the coin. Retrovirology 5:49
Pray L (2008) Restriction enzymes. Nat Educ 1(1):38
Raines RT (1998) Ribonuclease A. Chem Rev 98(3):1045–1066
Rittié L, Perbal B (2008) Enzymes used in molecular biology: a useful guide. J Cell Commun
Signal 2(1–2):25–45
Rogers SG, Weiss B (1980) Cloning of the exonuclease III gene of Escherichia coli. Gene
11:187–195
Saïda F, Odaert B, Uzan M, Bontems F (2004) First structural investigation of the restriction
ribonuclease RegB: NMR spectroscopic conditions, 13C/15N double-isotopic labelling and
two-dimensional heteronuclear spectra. Protein Expr Purif 34(1):158–165
Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988)
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
Science 239(4839):487–491
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc
Natl Acad Sci U S A 74:5463–5467
Stahl SJ, Zinn K (1981) Nucleotide sequence of the cloned gene for bacteriophage T7 RNA polymerase. J Mol Biol 148(4):481–485
Stein H, Hausen P (1969) Enzyme from calf thymus degrading the RNA moiety of DNA-RNA
hybrids: effect on DNA-dependent RNA polymerase. Science 166:393–395
Steitz TA (1998) A mechanism for all polymerases. Nature 391:231–232
Stenesh J, Roe B (1972) DNA polymerase from mesophilic and thermophilic bacteria. I. Purification
and properties from Bacillus licheniformis and Bacillus stearothermophilus. Biochim Biophys
Acta 272:167
Struhl K (1997) Enzymatic manipulation of DNA and RNA. In: Bensonchanda V (ed) Current
protocols in molecular biology. Wiley, Boston, pp 311–316
Tabor S, Richardson CC (1989) Selective inactivation of the exonuclease activity of bacteriophage
T7 DNA polymerase by in vitro mutagenesis. J Biol Chem 264:6447–6458
Tabor S, Huber HE, Richardson CC (1987) Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. J Biol Chem
262:16212–16223
Takagi M, Nishioka M, Kakihara H, Kitabayashi M, Inoue H, Kawakami B, Imanaka T (1997)
Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to
PCR. Appl Environ Microbiol 63(11):4504–4510
Takahashi K (1966) The structure and function of ribonuclease T1. VII. Further investigations
on amino acid composition and some other properties of ribonuclease T1. J Biochem 60:239
Talmadge K, Stahl S, Gilbert W (1980) Eukaryotic signal sequence transports insulin antigen in
Escherichia coli. Proc Natl Acad Sci U S A 77:3369–3373
Vanecko S, Laskowski M (1961) Studies of the specificity of deoxyribonuclease I. III. Hydrolysis
of chains carrying a monoesterified phosphate on carbon 5. J Biol Chem 236:3312–3316
Verjee ZHM (1969) Isolation of three acid phosphatases from wheat germ. Eur J Biochem
9(3):439–444
Wang LK, Lima CD, Shuman S (2002) Structure and mechanism of T4 polynucleotide kinase: an
RNA repair enzyme. EMBO J 21(14):3873–3880
4 Enzymes as Molecular Tools