Characterization of Gel Separated Proteins
87
Cowley S, Paterson H, Kemp P, Marshall CJ (1994) Activation of MAP kinase kinase is necessary and
sufficient for PC12 differentiation and for transformation of NIH3T3 cells. Cell 77: 841-852
Gheorghe MT, Jornvall H, Bergman T (1997) Optimized alcoholytic deacetylation ofN-acetyl-blocked
polypeptides for subsequent Edman degradation. Anal Biochem 254: 119-125
Henzel WJ, Billeci TM, Stults JT, Wong SC, Grimley C, Watanabe C (1993) Identifying proteins from
two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases. Proc Nat! Acad Sci USA 90: 5011-5015
Patterson SD (1994) From electrophoreticaily separated protein to identification: Strategies for
sequence and mass analysis. Anal Biochem 221: 1-15
Rasmussen RK, Ji H, Eddes JS, Moritz RL, Reid GE, Simpson RJ, Dorow DS (1997) Two-dimensional
electrophoretic analysis of human breast carcinoma proteins: Mapping of proteins that bind to the
SH3 domain of mixed lineage kinase MLK2. Electrophoresis 18: 588-598
Schevchenko A, Jensen ON, Podtelejnikov AV, Sagliocco F, Wilm M, Vorm 0, Mortensen P, Shevchenko A, Boucherie H, Mann M (1996) Linking genome and proteome by mass spectrometry:
Large-scale identification of yeast proteins from two dimensional gels. Proc Nat! Acad Sci USA 93:
14440-14445
Wilm M, Mann, M (1996) Analytical properties of the nanoelectrospray ion source. Anal Chern 68:
1-8
Wilm M, Schevchenko A, Houthaeve T, Breit S, Schweigerer L, Fotsis T, Mann M (1996) Femtomole
sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry. Nature
379: 466-469
87
Cowley S, Paterson H, Kemp P, Marshall CJ (1994) Activation of MAP kinase kinase is necessary and
sufficient for PC12 differentiation and for transformation of NIH3T3 cells. Cell 77: 841-852
Gheorghe MT, Jornvall H, Bergman T (1997) Optimized alcoholytic deacetylation ofN-acetyl-blocked
polypeptides for subsequent Edman degradation. Anal Biochem 254: 119-125
Henzel WJ, Billeci TM, Stults JT, Wong SC, Grimley C, Watanabe C (1993) Identifying proteins from
two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases. Proc Nat! Acad Sci USA 90: 5011-5015
Patterson SD (1994) From electrophoreticaily separated protein to identification: Strategies for
sequence and mass analysis. Anal Biochem 221: 1-15
Rasmussen RK, Ji H, Eddes JS, Moritz RL, Reid GE, Simpson RJ, Dorow DS (1997) Two-dimensional
electrophoretic analysis of human breast carcinoma proteins: Mapping of proteins that bind to the
SH3 domain of mixed lineage kinase MLK2. Electrophoresis 18: 588-598
Schevchenko A, Jensen ON, Podtelejnikov AV, Sagliocco F, Wilm M, Vorm 0, Mortensen P, Shevchenko A, Boucherie H, Mann M (1996) Linking genome and proteome by mass spectrometry:
Large-scale identification of yeast proteins from two dimensional gels. Proc Nat! Acad Sci USA 93:
14440-14445
Wilm M, Mann, M (1996) Analytical properties of the nanoelectrospray ion source. Anal Chern 68:
1-8
Wilm M, Schevchenko A, Houthaeve T, Breit S, Schweigerer L, Fotsis T, Mann M (1996) Femtomole
sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry. Nature
379: 466-469