52
L. CASTELLANOS-SERRA et al.: Reversible Negative Staining of Protein on Electrophoresis Gels
Fernandez-Patron, C., Castellanos-Serra, L., Hardy, E., Guerra, M., Estevez, E., Mehl, E., Frank, R.W.
(1998) Understanding the mechanism of the zinc-ion stains ofbiomacromolecules in electrophoresis gels: Generalization of the Reverse-staining technique. Electrophoresis 19:2398 - 2406
Fernandez-Patron, C., Castellanos-Serra, L., Rodriguez, P. (1992) Reverse-staining of sodium dodecylsulfate polyacrylamide gels by imidazole-zinc salts: Sensitive detection of unmodified proteins.
BioTechniques 12: 564-573
Fernandez-Patron, c., Hardy, E., Sosa, A., Seoane, J., Castellanos-Serra, L. (1995) Double staining of
Coomassie blue stained gels by imidazole-zinc Reverse-staining: sensitive detection of Coomassie
blue undetected proteins. Anal. Biochem. 224:263 - 269
Gevaert, K., Demos, H., Sklyarova, T., Vandekerckhove, J., Houthaeve, T. (1998) A peptide concentration and purification method for protein characterization in the subpicomole range using MALDIPSD sequencing. Electrophoresis 19: 909-017
Hardy, E., Pupo, E., Casalvilla, R., Sosa, A., Trujillo, L., Lopez, E., Castellanos-Serra, L. (1996) Negative
staining with zinc-imidazole of gel electrophoresis-separated nucleic acids by zinc-imidazole. Electrophoresis 17:1537-1541
Hardy, E., Pupo, E., Castellanos-Serra, L., Reyes, J., Fernandez-Patron, C. (1997) Sensitive Reversestaining of bacteriallipopolysacharides on polyacrylamide gels by using zinc and imidazole salts.
Anal. Biochem. 244:28-32
Hardy, E., Santana, H., Sosa, A., Hernandez, L., Fernandez-Patron, c., Castellanos-Serra, L. (1996)
Recovery of biologically active proteins detected with imidazole-SDS-zinc (Reverse-staining) on
sodium dodecylsulfate gels. Anal. Biochem. 240:150-152
Heukeshoven, J., Dernick, R. (1991) Presented at the Electrophoresis Forum' 91 (Munich), Forum
Communication 501-506
Jensen, O.N., Podtelejnikov, A.V., Mann, M. (1997) Identification of the components as simple protein
mixtures by high accuracy peptide mass mapping and database searching. Anal. Chern.
69:4741-4750
Lee, C., Levin, A., Branton, D. (1987) Cooper staining: A five-minute protein stain for sodium
dodecylsulfate-polyacrylamide gels. Anal. Biochem. 166:308-312
Matsui, N.M., Smith, D.M., Clauser, K.R., Fichmann, J., Andrews, L.E., Sullivan, C.M., Burlingame,
A.L., Epstein, L.B. (1997), Immobilized pH gradient two-dimensional gel electrophoresis and mass
spectrometric identification of cytokine-regulated proteins in ME-180 cervical carcinoma cells.
Electrophoresis, 18:409 -417
Moritz, R.L. & Simpson, R.J. (1992a) Application of capillary reversed-phase high-performance liquid
chromatography to high-sensitivity protein sequence analysis. J. Chromatogr. 599:119-130
Moritz, R.L. & Simpson, R.J. (1992b) Purification of proteins and peptides for sequence analysis using
micro column liquid chromatography. J. Microcol. Sep. 4:485-489
Moritz, R.L., Eddes, J.S., Reid, G.E., Simpson R.J. (1996b) S-pyridylethylation of intact polyacrylamide
gels and in situ digestion of electrophoretically separated proteins: A rapid mass spectrometric
method for identifying cysteine-containing peptides. Electrophoresis. 17:907-917.
Moritz, R.L., Eddes, J.S., Reid, G.E., Simpson, R.J. (1996a) Capillary HPLC: A method for protein isolation and peptide mapping. Methods. 6:213-226.
Ortiz, M.L., Calero, M., Fernandez-Patron, c., Castellanos, L., Mendez, E. (1992) Imidazole-SDS-Zinc
Reverse-staining of proteins in gels containing or not SDS and microsequence of individual
unmodified electroblotted proteins. FEBS Letters, 296:300-304
Reid, G.E., Rasmussen, R.K., Dorow, D.S., Simpson, R.J. (1998) Capillary column chromatography
improves sample preparation for mass spectrometric analysis: complete characterization of human
alpha- enolase from two-dimensional gels following in situ proteolytic digestion. Electrophoresis,
19:946-955
Scheler, c., Lamer, S., Pan, Z., Salnikov, J., Jungblut, P. (1998) Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrixassisted laser desorption/ionization-mass spectrometry. Electrophoresis 19:918-927
Zugaro, L.M., Reid G.E., Hong, Ji., Eddes J.S., Murphy, A.C., Burgess, A.W., SimpsonR.J. (1998) Characterization of rat brain stathmin isoforms by two-dimensional gel electrophoresis-matrix assisted
laser desorption/ionization and electrospray ionization-ion trap mass spectrometry. Electrophoresis, 19:867-876.
L. CASTELLANOS-SERRA et al.: Reversible Negative Staining of Protein on Electrophoresis Gels
Fernandez-Patron, C., Castellanos-Serra, L., Hardy, E., Guerra, M., Estevez, E., Mehl, E., Frank, R.W.
(1998) Understanding the mechanism of the zinc-ion stains ofbiomacromolecules in electrophoresis gels: Generalization of the Reverse-staining technique. Electrophoresis 19:2398 - 2406
Fernandez-Patron, C., Castellanos-Serra, L., Rodriguez, P. (1992) Reverse-staining of sodium dodecylsulfate polyacrylamide gels by imidazole-zinc salts: Sensitive detection of unmodified proteins.
BioTechniques 12: 564-573
Fernandez-Patron, c., Hardy, E., Sosa, A., Seoane, J., Castellanos-Serra, L. (1995) Double staining of
Coomassie blue stained gels by imidazole-zinc Reverse-staining: sensitive detection of Coomassie
blue undetected proteins. Anal. Biochem. 224:263 - 269
Gevaert, K., Demos, H., Sklyarova, T., Vandekerckhove, J., Houthaeve, T. (1998) A peptide concentration and purification method for protein characterization in the subpicomole range using MALDIPSD sequencing. Electrophoresis 19: 909-017
Hardy, E., Pupo, E., Casalvilla, R., Sosa, A., Trujillo, L., Lopez, E., Castellanos-Serra, L. (1996) Negative
staining with zinc-imidazole of gel electrophoresis-separated nucleic acids by zinc-imidazole. Electrophoresis 17:1537-1541
Hardy, E., Pupo, E., Castellanos-Serra, L., Reyes, J., Fernandez-Patron, C. (1997) Sensitive Reversestaining of bacteriallipopolysacharides on polyacrylamide gels by using zinc and imidazole salts.
Anal. Biochem. 244:28-32
Hardy, E., Santana, H., Sosa, A., Hernandez, L., Fernandez-Patron, c., Castellanos-Serra, L. (1996)
Recovery of biologically active proteins detected with imidazole-SDS-zinc (Reverse-staining) on
sodium dodecylsulfate gels. Anal. Biochem. 240:150-152
Heukeshoven, J., Dernick, R. (1991) Presented at the Electrophoresis Forum' 91 (Munich), Forum
Communication 501-506
Jensen, O.N., Podtelejnikov, A.V., Mann, M. (1997) Identification of the components as simple protein
mixtures by high accuracy peptide mass mapping and database searching. Anal. Chern.
69:4741-4750
Lee, C., Levin, A., Branton, D. (1987) Cooper staining: A five-minute protein stain for sodium
dodecylsulfate-polyacrylamide gels. Anal. Biochem. 166:308-312
Matsui, N.M., Smith, D.M., Clauser, K.R., Fichmann, J., Andrews, L.E., Sullivan, C.M., Burlingame,
A.L., Epstein, L.B. (1997), Immobilized pH gradient two-dimensional gel electrophoresis and mass
spectrometric identification of cytokine-regulated proteins in ME-180 cervical carcinoma cells.
Electrophoresis, 18:409 -417
Moritz, R.L. & Simpson, R.J. (1992a) Application of capillary reversed-phase high-performance liquid
chromatography to high-sensitivity protein sequence analysis. J. Chromatogr. 599:119-130
Moritz, R.L. & Simpson, R.J. (1992b) Purification of proteins and peptides for sequence analysis using
micro column liquid chromatography. J. Microcol. Sep. 4:485-489
Moritz, R.L., Eddes, J.S., Reid, G.E., Simpson R.J. (1996b) S-pyridylethylation of intact polyacrylamide
gels and in situ digestion of electrophoretically separated proteins: A rapid mass spectrometric
method for identifying cysteine-containing peptides. Electrophoresis. 17:907-917.
Moritz, R.L., Eddes, J.S., Reid, G.E., Simpson, R.J. (1996a) Capillary HPLC: A method for protein isolation and peptide mapping. Methods. 6:213-226.
Ortiz, M.L., Calero, M., Fernandez-Patron, c., Castellanos, L., Mendez, E. (1992) Imidazole-SDS-Zinc
Reverse-staining of proteins in gels containing or not SDS and microsequence of individual
unmodified electroblotted proteins. FEBS Letters, 296:300-304
Reid, G.E., Rasmussen, R.K., Dorow, D.S., Simpson, R.J. (1998) Capillary column chromatography
improves sample preparation for mass spectrometric analysis: complete characterization of human
alpha- enolase from two-dimensional gels following in situ proteolytic digestion. Electrophoresis,
19:946-955
Scheler, c., Lamer, S., Pan, Z., Salnikov, J., Jungblut, P. (1998) Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrixassisted laser desorption/ionization-mass spectrometry. Electrophoresis 19:918-927
Zugaro, L.M., Reid G.E., Hong, Ji., Eddes J.S., Murphy, A.C., Burgess, A.W., SimpsonR.J. (1998) Characterization of rat brain stathmin isoforms by two-dimensional gel electrophoresis-matrix assisted
laser desorption/ionization and electrospray ionization-ion trap mass spectrometry. Electrophoresis, 19:867-876.