Overproduction, Purification and Structural Studies
kDa M
9467
3020
TP30 1 2 3 4 5 6
275
..-514
Fig. 20.1. SDS-PAGE (15 %-discontinuous system) of crude extracts from E. coli pEt/TthS14 cultures,
which had been grown for 3 h at 37°C after induction with different concentrations of IPTG. Lane I:
0.5 mM IPTG, Lane 2: 1.0 mM IPTG, Lane 3: 1.5 mM IPTG, Lane 4: 2.0 mM IPTG, Lane 5: 2.5 mM
IPTG, Lane 6: 3.0 mM IPTG
1
2
3
M kOa
S143
Fig. 20.2. SDS-PAGE (15 %-discontinuous system) of crude extracts from E. coli pEtlld/TthSl4 cultures, which had been grown for 3 h at 37°C after induction with IPTG at different A600 .. Lane 1: A600
0.60, lane 2: A600 0.70, lane 3: A600 0.95, M: molecular weight markers. In all cases (a) corresponds to
50 Ilg and (b) to 100 Ilg of total protein. Staining of the gel was performed with Coomassie blue