250 J. J. CALVETE and L. SANZ: Structural Characterisation of Porcine Seminal Plasma Psp-I/Psp-1i
Dostalova Z, Calvete JJ, Sanz L, Hettel C, Riedel D, Einspanier R, Schonek c., Topfer-Petersen E (1994)
Irnmunolocalization and quantitation of spermadhesin aSFP in ejaculated, swim-up, and capacitated bull spermatozoa. Bioi Chern Hoppe-Seyler 375: 457-461
Dost:Hova Z, Calvete JJ, Sanz L, Topfer-Petersen E (1995) Boar spermadhesin AWN-I: oligosaccharide
and zona-pellucida binding characteristics. Eur J Biochem 230: 329-336
Einspanier R, Krause I, Calvete JJ, Topfer-Petersen E, Klostermeyer H, Karg H (1994) Bovine seminal
plasma aSFP: localization of disulphide bridges and detection of three different isoelectric forms.
FEBS Lett. 344: 61-64
Ensslin M, Vogel T, Calvete JJ, Thole HH, Schmidtke J, Matsuda T, Topfer-Petersen E (1998) Molecular
cloning and characterization ofP47, a novel boar sperm-associated zona pellucida-binding protein
homologous to a family of mammalian secretory proteins. Bioi Reprod 58: 1057 -1 064
Green DPL (1997) Three-dimensional structure of the zona pellucida. Rev Reprod 2: 147-156
Kudo K, Yonezawa N, Katsumata T, Aoki H, Nakano M (1998) Localization of carbohydrate chains of
pig sperm ligand in the glycoprotein ZPB of egg zona pellucida. Eur J Biochem 252: 492-499
Lillie FR (1913) The mechanism offertilization. Science 38: 524-528
Reinert M, Calvete JJ, Sanz L, Mann K, Topfer-Petersen E (1996) Primary structure of stallion seminal
plasma protein HSP-7, a zona pellucida binding protein of the spermadhesin family. Eur J Biochem
242: 636-640
Romero A, Romao MJ, Varela PF, Kolin I, Dias JM, Carvalho AL, Sanz L, Topfer-Petersen E, Calvete JJ
(1997) The crystal structures of two spermadhesins reveal the CUB domain fold. Nature Struc Bioi
4: 783-788
Sinowatz F, Topfer-Petersen E, Calvete JJ (1997) Glycobiology of fertilization. In: Gabius H-J, Gabius
S (eds) Glycosciences: status and perspectives. Chapman and Hall, Weinheim, pp 595-610
Solis D, Calvete JJ, Sanz L, Hettel C, Raida M, Diaz-Mauriiio T & Topfer-Petersen E (1997) Fractionation and characterization of boar seminal plasma spermadhesin PSP-II glycoforms reveal the
presence of uncommon N-acetylgalactosamine-containing N-linked oligosaccharides. Glycoconjugate J 14: 275-280
Solis D, Romero A, Jimenez M, Diaz-Mauriiio T, Calvete JJ (1998) Binding of mannose-6-phosphate
and heparin by boar seminal plasma PSP-II, a membr of the spermadhesin protein family. FEBS
Lett 431: 273-278
Spijker NM, Keuning CA, Hooglut M, Veenernan GH, van Boekel CAA (1996) Synthesis of a hexasaccharide corresponding to a zona pellucida fragment that inhibits porcine sperm-oocyte interaction
in vitro. Tetrahedron 52: 5945-5069
Topfer-Petersen E, Calvete JJ (1996) Sperm-associated protein candidates for primary zona pellucidabinding molecules: structure-function correlations of boar spermadhesins. J Reprod Fertil Suppl
50: 55-61
Topfer-Petersen E, Romero A, Varela PF, Ekhlasi-Hundrieser M, Dostalova Z, Sanz L, Calvete JJ (1998)
Spermadhesins: a new protein family. Facts, hypotheses and perspectives. Andrologia 30: 217 - 224
Trabesinger-RuefN, Jermann T, Zankel T, Durrant B, Frank G, Benner SA (1996) Pseudogenes in ribonuclease evolution; a source of new biomolecular function? FEBS Lett 382: 319-322
Tulsiani DRP, Yoshida-Komiya H, Araki Y (1997) Mammalian fertilization: a carbohydrate-mediated
event. Bioi Reprod 57: 487 -494
Varela PF, Romero A, Sanz L, Romao MJ, Topfer-Petersen E, Calvete JJ (1997) The 2.4 A resolution
crystal structure of boar seminal plasma PSP-I/PSP-II: a zona pellucida-binding glycoprotein heterodimer of the spermadhesin family built by a CUB domain architecture. J Mol Bioi 274: 635-649
Veselsky J, Dostal J, Holan V, Soucek J, Zelezna B (1996) Effect of boar seminal immunosuppressive
fraction on B-Iymphocyte and on primary antibody response. Bioi Reprod 55: 194-199
Yang WC, Kwok SCM, Leshin S, Bolio E, Li WI (1998) Purified porcine seminal plasma protein
enhances in vitro immune activities of porcine peripheral lymphocytes. Bioi Reprod 59: 202-207
Yurewicz EC, Pack BA, Sacco AG (1991) Isolation, composition, and biological activity of sugar chains
of porcine oocyte zona pellucida 55K glycoproteins. Mol Reprod Dev 30: 126-134
Dostalova Z, Calvete JJ, Sanz L, Hettel C, Riedel D, Einspanier R, Schonek c., Topfer-Petersen E (1994)
Irnmunolocalization and quantitation of spermadhesin aSFP in ejaculated, swim-up, and capacitated bull spermatozoa. Bioi Chern Hoppe-Seyler 375: 457-461
Dost:Hova Z, Calvete JJ, Sanz L, Topfer-Petersen E (1995) Boar spermadhesin AWN-I: oligosaccharide
and zona-pellucida binding characteristics. Eur J Biochem 230: 329-336
Einspanier R, Krause I, Calvete JJ, Topfer-Petersen E, Klostermeyer H, Karg H (1994) Bovine seminal
plasma aSFP: localization of disulphide bridges and detection of three different isoelectric forms.
FEBS Lett. 344: 61-64
Ensslin M, Vogel T, Calvete JJ, Thole HH, Schmidtke J, Matsuda T, Topfer-Petersen E (1998) Molecular
cloning and characterization ofP47, a novel boar sperm-associated zona pellucida-binding protein
homologous to a family of mammalian secretory proteins. Bioi Reprod 58: 1057 -1 064
Green DPL (1997) Three-dimensional structure of the zona pellucida. Rev Reprod 2: 147-156
Kudo K, Yonezawa N, Katsumata T, Aoki H, Nakano M (1998) Localization of carbohydrate chains of
pig sperm ligand in the glycoprotein ZPB of egg zona pellucida. Eur J Biochem 252: 492-499
Lillie FR (1913) The mechanism offertilization. Science 38: 524-528
Reinert M, Calvete JJ, Sanz L, Mann K, Topfer-Petersen E (1996) Primary structure of stallion seminal
plasma protein HSP-7, a zona pellucida binding protein of the spermadhesin family. Eur J Biochem
242: 636-640
Romero A, Romao MJ, Varela PF, Kolin I, Dias JM, Carvalho AL, Sanz L, Topfer-Petersen E, Calvete JJ
(1997) The crystal structures of two spermadhesins reveal the CUB domain fold. Nature Struc Bioi
4: 783-788
Sinowatz F, Topfer-Petersen E, Calvete JJ (1997) Glycobiology of fertilization. In: Gabius H-J, Gabius
S (eds) Glycosciences: status and perspectives. Chapman and Hall, Weinheim, pp 595-610
Solis D, Calvete JJ, Sanz L, Hettel C, Raida M, Diaz-Mauriiio T & Topfer-Petersen E (1997) Fractionation and characterization of boar seminal plasma spermadhesin PSP-II glycoforms reveal the
presence of uncommon N-acetylgalactosamine-containing N-linked oligosaccharides. Glycoconjugate J 14: 275-280
Solis D, Romero A, Jimenez M, Diaz-Mauriiio T, Calvete JJ (1998) Binding of mannose-6-phosphate
and heparin by boar seminal plasma PSP-II, a membr of the spermadhesin protein family. FEBS
Lett 431: 273-278
Spijker NM, Keuning CA, Hooglut M, Veenernan GH, van Boekel CAA (1996) Synthesis of a hexasaccharide corresponding to a zona pellucida fragment that inhibits porcine sperm-oocyte interaction
in vitro. Tetrahedron 52: 5945-5069
Topfer-Petersen E, Calvete JJ (1996) Sperm-associated protein candidates for primary zona pellucidabinding molecules: structure-function correlations of boar spermadhesins. J Reprod Fertil Suppl
50: 55-61
Topfer-Petersen E, Romero A, Varela PF, Ekhlasi-Hundrieser M, Dostalova Z, Sanz L, Calvete JJ (1998)
Spermadhesins: a new protein family. Facts, hypotheses and perspectives. Andrologia 30: 217 - 224
Trabesinger-RuefN, Jermann T, Zankel T, Durrant B, Frank G, Benner SA (1996) Pseudogenes in ribonuclease evolution; a source of new biomolecular function? FEBS Lett 382: 319-322
Tulsiani DRP, Yoshida-Komiya H, Araki Y (1997) Mammalian fertilization: a carbohydrate-mediated
event. Bioi Reprod 57: 487 -494
Varela PF, Romero A, Sanz L, Romao MJ, Topfer-Petersen E, Calvete JJ (1997) The 2.4 A resolution
crystal structure of boar seminal plasma PSP-I/PSP-II: a zona pellucida-binding glycoprotein heterodimer of the spermadhesin family built by a CUB domain architecture. J Mol Bioi 274: 635-649
Veselsky J, Dostal J, Holan V, Soucek J, Zelezna B (1996) Effect of boar seminal immunosuppressive
fraction on B-Iymphocyte and on primary antibody response. Bioi Reprod 55: 194-199
Yang WC, Kwok SCM, Leshin S, Bolio E, Li WI (1998) Purified porcine seminal plasma protein
enhances in vitro immune activities of porcine peripheral lymphocytes. Bioi Reprod 59: 202-207
Yurewicz EC, Pack BA, Sacco AG (1991) Isolation, composition, and biological activity of sugar chains
of porcine oocyte zona pellucida 55K glycoproteins. Mol Reprod Dev 30: 126-134